Protein Info for PS417_14490 in Pseudomonas simiae WCS417

Annotation: 4-alpha-glucanotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 694 TIGR00217: 4-alpha-glucanotransferase" amino acids 114 to 666 (553 residues), 356.2 bits, see alignment E=2e-110 PF02446: Glyco_hydro_77" amino acids 158 to 663 (506 residues), 375.4 bits, see alignment E=2.3e-116

Best Hits

KEGG orthology group: K00705, 4-alpha-glucanotransferase [EC: 2.4.1.25] (inferred from 94% identity to pfs:PFLU3366)

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TXU1 at UniProt or InterPro

Protein Sequence (694 amino acids)

>PS417_14490 4-alpha-glucanotransferase (Pseudomonas simiae WCS417)
MSEANLEILASRAGLAVDWIDANGRPQRVKPDALRAVLKGLGHPADSDAEVDASLLELER
AQQDTHLPPLMTIDSGEGLDLARYFEPDTLCRVSLEDGETLDLRLDGDAVLPGVIALGYH
QVHIAEQTFTLAVAPTHCYSVAEAVDSRPARAWGLSAQLYSLRRLGDGGFGDTLALEHLA
RSAAERGADALAISPMHAMFSADTERYSPYSPSSRLFLNSLYASPGCILGEREVRNAIDA
LGLTDELHDLEQHTLIDWPTAAKAKQRLLRALYEDFRHGQHPQHADFLSFRQAGGEALEN
HCRFEAVQAARAAKGEDLDWRHWPEDWRNPQSPALVAFAAANADEIGYFAFSQWLIARCL
ERAQQAARGSGMGVGLIADLAVGADGGGSQAWSRQDELLANLTVGAPPDILNRAGQGWGI
SAFSPEGLKRHGFRAFIEMLRANFAHAGGLRIDHVMGLQRLWVIPMGASPREGAYLYYPV
DDLLRLLALESHRHHAIVLGEDLGTVPDGLREKLIARSILGMRVLLFEQNHDGQFKPILD
WPDNALATTSTHDLPTLNGWWHSRDIDWNVQLGLIDAPTVEQWSEHRLRERQALRQALSQ
DPQNFVEEIRNETDHMIDASVRYLGHTRAPLVLLPLEDALGVEEQANLPGTTDTHPNWRR
RLPGEASSLLDNAGAARRLELLAVARNQAYERDR