Protein Info for PS417_14485 in Pseudomonas simiae WCS417

Annotation: malto-oligosyltrehalose trehalohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 581 TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 21 to 545 (525 residues), 685.2 bits, see alignment E=2.7e-210 PF00128: Alpha-amylase" amino acids 125 to 208 (84 residues), 47 bits, see alignment E=2.4e-16 PF11941: DUF3459" amino acids 490 to 572 (83 residues), 59.5 bits, see alignment E=3.5e-20

Best Hits

KEGG orthology group: K01236, maltooligosyltrehalose trehalohydrolase [EC: 3.2.1.141] (inferred from 92% identity to pfs:PFLU3365)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7ULJ2 at UniProt or InterPro

Protein Sequence (581 amino acids)

>PS417_14485 malto-oligosyltrehalose trehalohydrolase (Pseudomonas simiae WCS417)
MPLRTLETWPHGAIMLDAQHTRFALWAPDAFYVSVELENGKSVAMLPQAEGWFETEVACP
AGTRYRFNIDGEMDVPDPASRAQASDVHGWSVVVDPLAYQWRHSQWQGRPWHEAVIYELH
VGAMGGYAAVEKHLPRLAELGVTAIELMPLAQFPGERNWGYDGVLPYAPQASYGTPDQLK
HLIDSAHEHGLAVILDVVYNHFGPDGNYLGQYAKGFFQEDVHTPWGAGIDFDRREVRDFF
LDNALMWLLEYRFDGLRLDAVHAIDNPDFLKQLAHRVREQVDTGRHVWLMLENELNQASL
LQHDFDAQWNDDFHNVLHVLLTGETDAYYSDFAQAPTAKLARCLGEGFIYQGHTTRHGHE
RGEPSGHLPPSAFVAFLQNHDQIGNRALGERLHQLCSPQALEAATALLLLSPMIPLMFMG
DEVNASEPFLFFTDHHGELAEAVREGRRNEFADFAAFHDPQRRERIPDPNALATFQQSAP
TFADHPHAQLYRQLLNLRHHHIVPHVPGSLALGAQVLADGAVSARWRLGNGSLLQIDLNL
SATPLDHPAAGPVLFETPANLGAHLPPYSARVTLSPVGEHP