Protein Info for GFF2824 in Sphingobium sp. HT1-2

Annotation: alpha-L-fucosidase (EC 3.2.1.51)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 541 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01120: Alpha_L_fucos" amino acids 30 to 428 (399 residues), 258.5 bits, see alignment E=6.2e-81

Best Hits

KEGG orthology group: K01206, alpha-L-fucosidase [EC: 3.2.1.51] (inferred from 58% identity to cak:Caul_1302)

Predicted SEED Role

"Alpha-L-fucosidase (EC 3.2.1.51)" in subsystem L-fucose utilization or L-fucose utilization temp (EC 3.2.1.51)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (541 amino acids)

>GFF2824 alpha-L-fucosidase (EC 3.2.1.51) (Sphingobium sp. HT1-2)
MQDFGRRSFLAGAAALGLANAARAASPMGTAKGPVQASWPSLVDNYRYPDWFRDAKLGMW
SHWGPQSVPAQGDWYGRFMYMQGHPMYEHHLKTYGHPSVAGMKDIQNLWTADKWDPDALM
AKYQKAGAKYFMALACHHDNLDCYDSRYHAWNSLRVGPKKDIVGIWEKAARKAGLKIGVS
NHAAHAWHWYQPAYGYDPVGPQKGVRYDAFRLRKEDGKGQWWDGLDPRELYTGGHAVLPD
GIDTIEAMDKWHDANNGQWIETGPKDDPAYVTRWLLRQTDLVAKYKPDMVYMDDHELPFG
PVGLEAAADYYNRSIEWHGKIDVVMTGKQLKPYARFGIVQDVERGYTDHIWDEPWQTDTC
IGDWFYNIARLTDKSYKSAEQVIQRLADVVSKNGNLMLSIPQPGDGSIDSEAEKILDAMA
GWMVHGGEAIFGSRPWKRYGEGPTQAIVGMQNEEKAKPFTAQDIRFTTNKGALYALFLGK
PAGSTTIRSLARGQGTGTIERVTLLGGGQLAFRQDGAGLHFTMPHGIDFVPAVRIDGRGL
V