Protein Info for GFF2812 in Sphingobium sp. HT1-2

Annotation: 2-keto-3-deoxy-L-fuconate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 247 PF00106: adh_short" amino acids 12 to 191 (180 residues), 148.6 bits, see alignment E=3.2e-47 PF08659: KR" amino acids 13 to 154 (142 residues), 24.1 bits, see alignment E=6.4e-09 PF13561: adh_short_C2" amino acids 19 to 245 (227 residues), 184.9 bits, see alignment E=3.6e-58 PF23441: SDR" amino acids 123 to 244 (122 residues), 28.7 bits, see alignment E=1.7e-10

Best Hits

Swiss-Prot: 54% identical to BDH2_RAT: 3-hydroxybutyrate dehydrogenase type 2 (Bdh2) from Rattus norvegicus

KEGG orthology group: None (inferred from 67% identity to cak:Caul_1544)

MetaCyc: 62% identical to 2-dehydro-3-deoxy-D-pentonate/2-dehydro-3-deoxy-L-fuconate 4-dehydrogenase (Herbaspirillum huttiense)
RXN-22641 [EC: 1.1.1.434]; 1.1.1.434 [EC: 1.1.1.434]

Predicted SEED Role

"2-keto-3-deoxy-L-fuconate dehydrogenase" in subsystem L-fucose utilization temp

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.434

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (247 amino acids)

>GFF2812 2-keto-3-deoxy-L-fuconate dehydrogenase (Sphingobium sp. HT1-2)
MSAPQNGRLAGKTALITAAARGIGRASVERFVAEGARVFATDRDLDALDGLEGCERLALD
VTDGAAVRALAAEIGPIDILANIAGVVHAGNILECSQSDWDFAVALNMTAQYHTISAFLP
GMVEKGAGAIVNMSSIASSVKGIPNRFAYGATKAGVIGLTKAVAADFVGQGIRCNCICPG
TVDTPSLQQRLHDTGDYEAAQAAFVARQPMGRLGKAQEIAGLVLYLASDDAAFTTGAVHV
IDGGWVM