Protein Info for GFF2808 in Sphingobium sp. HT1-2

Annotation: Gluconolactonase (EC 3.1.1.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12708: Pect-lyase_RHGA_epim
  • amino acids 41 to 265 (225 residues), 79 bits, E=7e-26
  • amino acids 380 to 433 (54 residues), 42 bits, 1.4e-14
• PF08450: SGL
  • amino acids 911 to 999 (89 residues), 32.1 bits, E=1.4e-11

Best Hits

KEGG orthology group: None (inferred from 73% identity to cse:Cseg_1861)

Predicted SEED Role

"Gluconolactonase (EC 3.1.1.17)" in subsystem Entner-Doudoroff Pathway (EC 3.1.1.17)

MetaCyc Pathways

• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• glucose and glucose-1-phosphate degradation (4/5 steps found)
• sorbitol biosynthesis II (2/3 steps found)
• L-ascorbate biosynthesis IV (animals, D-glucuronate pathway) (3/6 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (5/9 steps found)
• glucose degradation (oxidative) (2/5 steps found)
• L-ascorbate biosynthesis VI (plants, myo-inositol pathway) (1/4 steps found)
• L-ascorbate biosynthesis VIII (engineered pathway) (2/7 steps found)

KEGG Metabolic Maps

• Ascorbate and aldarate metabolism
• Caprolactam degradation
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.17

Use Curated BLAST to search for 3.1.1.17

Protein Sequence (1007 amino acids)

>GFF2808 Gluconolactonase (EC 3.1.1.17) (Sphingobium sp. HT1-2)
MIGRSMIAALLAATTLGAPAFAATTSVFPVAPAEPHAVTVKAVGDGRADDSAAIQQALDQ
ARDATGHGIVFLPSGTYRITRSLIVPAGVRVYGVGPTRPVLLLGANTPGFQQGVSTMVIF
AGGDQYQVGKVPVPVPTVVPRDKIVRDANSGTFYSSMSNVDIEIGAGNPAAAGVRFRMAQ
HAFLSHMEFRLGTAFAGVYQAGNVIENVHFQGGRYGIVTEKTSPAWQFTLLDSSFDGQRD
AAIREHEVDLTLVNVAIKNTPVGIEIDRGYSDSLWGKDVRFENVSKAGVVISNEKNVFTQ
VGFDNALAVNSPVFARFRDSGRTIDGKGKAYKVANFSYGLAVPALGHTGEYATTADIQPL
SAMPAPRASAIRDLPPMDQWVNVRTLGAVGDGKADDTAALQKAIDANRILYFPTGFYKVT
DRLTLRPDSILIGLHPAITQLFIPDNNPKHAGLGAVLPILESRKGGDNILSGLGLFTGRV
NPRASALLWRSGEQSLVEDVKIMGGGGTPTADGKMLGTLRVNTGDPVTDSRLDAQYPSIW
VTDGGGGTFADVWSPNSFAQAGFYITDTDTPGHIYEMSVEHHARNEFVLDNVHNWEFLAP
QTEQEVDDGPDAISLDIRNSSNLLFANYHGYRVTRTYAPEKSAVKITNSGNIRFRNVHVN
GESGYATCDDEGCGTFLRASKYPFDNAIEDVSRKLLVREREFAALDIGPAGSAVPALAPS
GTKVEKLEDGFWSISGAAVDAQGQLYFIDRRFQRIHRWSEGKGLEIVRDHALDPVNLAID
ASGHVMVLSSLGAKGGAYSFDPAGPKDALTLIQPTPVRSTGAAKTLLPVNWWNNGEFRDQ
LDPKSYEFTTLAEMFARDVGTPKAKEYLSPDGSLSLPAFRVWQQGPIDHTGWRWSDGLNA
NGFVSGKIGDRLFVTNGSENITYSGTVGAGGTLTALKPFANRGGESVAVDGQGRVFVANG
QIFVYGADGKEAGRIDVPDRPLQILFGGPDKRTLFILTHHALYAAKP