Protein Info for GFF2806 in Sphingobium sp. HT1-2

Annotation: Gluconolactonase (EC 3.1.1.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12708: Pect-lyase_RHGA_epim
  • amino acids 44 to 290 (247 residues), 71.8 bits, E=7.6e-24
  • amino acids 384 to 507 (124 residues), 53 bits, E=4.1e-18
• PF08450: SGL
  • amino acids 913 to 1003 (91 residues), 37.5 bits, E=2.2e-13

Best Hits

KEGG orthology group: None (inferred from 71% identity to cak:Caul_2072)

Predicted SEED Role

"Gluconolactonase (EC 3.1.1.17)" in subsystem Entner-Doudoroff Pathway (EC 3.1.1.17)

MetaCyc Pathways

• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• glucose and glucose-1-phosphate degradation (4/5 steps found)
• sorbitol biosynthesis II (2/3 steps found)
• L-ascorbate biosynthesis IV (animals, D-glucuronate pathway) (3/6 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (5/9 steps found)
• glucose degradation (oxidative) (2/5 steps found)
• L-ascorbate biosynthesis VI (plants, myo-inositol pathway) (1/4 steps found)
• L-ascorbate biosynthesis VIII (engineered pathway) (2/7 steps found)

KEGG Metabolic Maps

• Ascorbate and aldarate metabolism
• Caprolactam degradation
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.17

Use Curated BLAST to search for 3.1.1.17

Protein Sequence (1012 amino acids)

>GFF2806 Gluconolactonase (EC 3.1.1.17) (Sphingobium sp. HT1-2)
MYRGLSGACASVLLCLAAAQPAFASPSVYTAMPDDARAVTVKGVGDGRADDSAAIQQALD
AAAATPGGGIVFLPQGTYRISRTLFLWPGVRIFGIGAQRPVILLGANTPGFQRGVAHMLI
FTGGKRQTDKDSWPAAFPPAGSVPFDKTIADANPGTFYSALGNIDFRIMDGNPAATAIRF
HSAQHSFVSHVDFDIGSGLAGLYHVANEAEDLHFKGGRYGILAEKTSPAWPFALVDATFE
GQRDAAIREHEAGLTLLNVAFRNVPVGIEIDRGYGDWLWGQDVRFENVSKAGVIISNEKN
VYTQVGFQNVLAANTPTFARFRESGRTVAGQGKTYKVTSFTHGLTLPGLGRIGDYKTDMQ
AQPIASLPAPGAPAIRPLPPVAEWVNVRTLGAKGDNSTDDTAVLQKAIDSHRVVYLPAGF
YKVSDTLRLRADTVLIGLHPSLTQIVLPDGTPAFQGVGAPKALIESAQGGDAIVAGLALD
TNGANPRATGLLWKAGANSMVNDVKFQGGHGTDAFDGSRRSPYNNNATADPDASRRWGGQ
YASLWVTQGGGGTFANIWSPSTFGHPGILISDTDPPGRLIQASVEHHVRTEIGLNRVANW
ELLAPQTEGEAGESGDAVALEIRDSRNILVANFHGYRVTRTRKPAPTAVSLYNSHDIRFR
NVHVNAESGVGTCDENGCGTFLRLTKYPFENAIQDITHGLEVREREFAVLDVPAQPDPVT
PSTFGGASVEKIADGFYGLGGGAVDAQGRLYFIDRFFQRIWRWSDAGRLEMIRDAALDPV
NLTIDKSGNLLVLSSYGRNGTVYSVTPGAPDTQVSVIPATPAGQGAAATIFPVNWWVNGE
FRDQIDPKTYAFTTLAEMFARDTAQPNAQAYVSPDGSIALPAWRVFAQGPVDHRGLRFSH
SLDSYGFVQGKPGQRIYVTNGSENRTYSALVAGNGTLIDLKPFADRGGEGVVSDAQGRVY
VANGQVFVYAPDGKPIGRIDVPERPLQLLFGGPDGKTLFMLSHHGLYAARVE