Protein Info for GFF2800 in Variovorax sp. SCN45

Annotation: Threonine synthase (EC 4.2.3.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14821: Thr_synth_N
  • amino acids 2 to 79 (78 residues), 101 bits, E=4.9e-33
• TIGR00260: threonine synthase
  • amino acids 68 to 433 (366 residues), 230.8 bits, E=1.2e-72
• PF00291: PALP
  • amino acids 92 to 331 (240 residues), 44.9 bits, E=1.6e-15
• PF24857: THR4_C
  • amino acids 396 to 467 (72 residues), 31.6 bits, E=2.2e-11

Best Hits

Swiss-Prot: 64% identical to THRC_METGL: Threonine synthase (thrC) from Methylobacillus glycogenes

KEGG orthology group: K01733, threonine synthase [EC: 4.2.3.1] (inferred from 94% identity to vap:Vapar_1832)

Predicted SEED Role

"Threonine synthase (EC 4.2.3.1)" in subsystem Threonine and Homoserine Biosynthesis (EC 4.2.3.1)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• L-threonine biosynthesis (2/2 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Vitamin B6 metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.3.1

Use Curated BLAST to search for 4.2.3.1

Protein Sequence (471 amino acids)

>GFF2800 Threonine synthase (EC 4.2.3.1) (Variovorax sp. SCN45)
VNYLSTRGHPDRKRFCDILLEGLAPDGGLYLPEQYPKVDTATLAKWRDLPYAELAFEILS
LYIDDIPAADLKALCAKTYTAEVFGSDEIVPLRELEDGVYLEALSNGPTLAFKDMAMQLL
GNLFEYELGRRGAELNILGATSGDTGSAAEYAMRGKKGVRVFMTSPDGRMSPFQQAQMFS
LQDENIHNIAITGVFDDCQDIVKAVSNDLGFKRKYRIGTVNSINWARLLAQVVYYFAGYF
QATASNDKPVSFTVPSGNFGNVCAGHVARMMGLPIQTLVVATNENDVLDEFFRTGVYRVR
GAADTHETSSPSMDISKASNFERFVFDLVGRDAAKLRKLFVEDLGLQGSFDLSGDPVFKQ
AAERFGFKSSRSTHADRLAVIRDTDKRFATLVDPHTADGLKAAREHLAPGVPMVVLETAL
PIKFAETLVEALGEEPARPKKFEGIEALPKRVVKLPADAEAVKAYIAQNCD