Protein Info for PS417_14260 in Pseudomonas simiae WCS417

Annotation: phosphate ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 transmembrane" amino acids 26 to 51 (26 residues), see Phobius details amino acids 83 to 108 (26 residues), see Phobius details amino acids 122 to 144 (23 residues), see Phobius details amino acids 151 to 171 (21 residues), see Phobius details amino acids 195 to 217 (23 residues), see Phobius details amino acids 236 to 259 (24 residues), see Phobius details amino acids 270 to 292 (23 residues), see Phobius details TIGR00974: phosphate ABC transporter, permease protein PstA" amino acids 28 to 294 (267 residues), 262.7 bits, see alignment E=1.7e-82 PF00528: BPD_transp_1" amino acids 99 to 292 (194 residues), 54.2 bits, see alignment E=8.2e-19

Best Hits

Swiss-Prot: 43% identical to PSTA_ECOLI: Phosphate transport system permease protein PstA (pstA) from Escherichia coli (strain K12)

KEGG orthology group: K02038, phosphate transport system permease protein (inferred from 99% identity to pfs:PFLU3316)

MetaCyc: 43% identical to phosphate ABC transporter membrane subunit PstA (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate transport system permease protein PstA (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UFS4 at UniProt or InterPro

Protein Sequence (297 amino acids)

>PS417_14260 phosphate ABC transporter permease (Pseudomonas simiae WCS417)
MTDLSTPIVAMPSLQRRFEGRALRSLVLTTLVWFGALLAAVPLISVLYMLITRGGARLSL
EVFTELPPTGFETGGGFGNAMAGTFVMVGIAAAIAVPVGILAAIFLAELGPDSKLANASR
FAAKMLTGLPSILAGVFAYALVVMTTGTYSAPAGGVALAVLMLPIVVLTAEESMKMVPKI
MKDAAYGMGCTRSQVIWKIVLPTGMPAILTGVMLAVARAAGETAPLLFTALFSNYWIYHD
GSLAVMNPTASLAVLIYNFSGMPFDNQLELAWAASLVLVMIVLVVNIVSRIFGKPKY