Protein Info for GFF279 in Xanthobacter sp. DMC5

Annotation: Allophanate hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01425: Amidase
  • amino acids 24 to 431 (408 residues), 296 bits, E=5.8e-92
• TIGR02713: allophanate hydrolase
  • amino acids 36 to 590 (555 residues), 761.6 bits, E=2.5e-233
• PF21986: AH_C
  • amino acids 470 to 590 (121 residues), 151 bits, E=1.6e-48

Best Hits

KEGG orthology group: K01457, allophanate hydrolase [EC: 3.5.1.54] (inferred from 84% identity to xau:Xaut_4705)

MetaCyc: 49% identical to allophanate hydrolase (Rhizobium leguminosarum bv. viciae 3841)
Allophanate hydrolase. [EC: 3.5.1.54]

Predicted SEED Role

"Allophanate hydrolase (EC 3.5.1.54)" in subsystem Urea decomposition (EC 3.5.1.54)

MetaCyc Pathways

• cyanuric acid degradation II (5/5 steps found)
• L-citrulline degradation (3/3 steps found)
• cyanate degradation (3/3 steps found)
• urea degradation I (3/3 steps found)
• cyanuric acid degradation I (4/5 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• superpathway of allantoin degradation in yeast (4/6 steps found)
• allantoin degradation IV (anaerobic) (6/9 steps found)
• uracil degradation III (3/5 steps found)
• superpathway of atrazine degradation (5/8 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Atrazine degradation
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.54

Use Curated BLAST to search for 3.5.1.54

Protein Sequence (594 amino acids)

>GFF279 Allophanate hydrolase (Xanthobacter sp. DMC5)
MQTVASLLEAHRSGLRSVSDTVRGVYARLAVHDDPAMFITLREEADVLAEAAALETAGNT
DLPLYGIPVAVKDNIDVAGLPTTAACPAFAYVPERDATVVARLKAAGALIIGKTNLDQFA
TGLVGVRSPYGIPRNSVNPELVPGGSSSGSAVAVGAGIVPVSLGTDTAGSGRVPAMLNNI
VGLKPSLGLVPNTGLVPACKTLDCISIFALTVDDAMAVLEVIAGFDEQDAYSRDLPVGPL
TAAPPAPVLGILPEAQREFFGDAESAAAYEEALARFASLGAVLKEIDFSAFAETARLLYE
GPWVAERWIAAEHLLTTDPDAVHPVTRAITEPGGKASAADAFRAMYRLKELRARVAPILA
GIDALLLPTAPSAYTVEEVLADPIRLNSRNGTYTNFVNLMDLCGIAVPTKITAAGVPYGI
TLLAPAGRDAEMAALGRAYVAAGNLPLGCGHAPAPELPPLAPASEPGRLDVCVFGAHLSG
MPLNGDLKAMGARFVRAAKTVPSYRMKLLPGAIPRPGVVRVTADGAAVEGEVWSLPFEGV
GRLLATIPAPLGLGQVELDDGSRVTGFLAEAAALEGARDITEFGSFRAFAAAAE