Protein Info for GFF2785 in Sphingobium sp. HT1-2

Annotation: Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) / Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 7 to 122 (116 residues), 135.5 bits, E=1e-43
• PF02882: THF_DHG_CYH_C
  • amino acids 125 to 287 (163 residues), 215.6 bits, E=2.4e-68

Best Hits

Swiss-Prot: 75% identical to FOLD3_SPHWW: Bifunctional protein FolD 3 (folD3) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 89% identity to sch:Sphch_2828)

MetaCyc: 56% identical to methylenetetrahydrofolate dehydrogenase [multifunctional] (Bacillus methanolicus)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations III (E. coli) (8/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• folate transformations II (plants) (8/11 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• L-histidine degradation III (4/6 steps found)
• folate transformations I (8/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Protein Sequence (297 amino acids)

>GFF2785 Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) / Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) (Sphingobium sp. HT1-2)
MTIGKIIDGKAFAAGLRDKVADGVAAFVEQTGRKPGLAVVLVGEDPASSVYVRNKGKMTL
AAGMESFEFKRPDSIGEDDLLDLIEELNHDDRVDGILVQLPLPRHIDEAAVIAAIDPAKD
VDGFHVVNTGRLATGQDALVPCTPLGCIMLLKDELGDLSGMEAVVVGRSNIVGKPMAALL
LAENCTVTIAHSRTRDIASIVHRADIVVAAVGRAEMVKGEWIKPGATVIDVGINRVVDTE
DGEGRIVGDVATAEALSHVRAITPVPGGVGPMTIAVLLRNTLVAAHARAGLAKPEGL