Protein Info for GFF2774 in Xanthobacter sp. DMC5

Annotation: Putative 3-oxopropanoate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)
  • amino acids 5 to 480 (476 residues), 540.5 bits, E=1.8e-166
• PF00171: Aldedh
  • amino acids 19 to 476 (458 residues), 422.7 bits, E=8.1e-131

Best Hits

Swiss-Prot: 52% identical to BAUC_PSEAE: Putative 3-oxopropanoate dehydrogenase (bauC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 71% identity to swi:Swit_4138)

MetaCyc: 47% identical to malonate-semialdehyde dehydrogenase (acetylating) (Halomonas sp. HTNK1)
Malonate-semialdehyde dehydrogenase (acetylating). [EC: 1.2.1.18]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

• β-alanine degradation II (2/2 steps found)
• β-alanine degradation I (1/2 steps found)
• acrylate degradation I (3/5 steps found)
• propanoyl-CoA degradation II (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• 2,4-dinitrotoluene degradation (3/7 steps found)
• myo-inositol degradation I (2/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (2/10 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Inositol phosphate metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.27

Use Curated BLAST to search for 1.2.1.18 or 1.2.1.27

Protein Sequence (499 amino acids)

>GFF2774 Putative 3-oxopropanoate dehydrogenase (Xanthobacter sp. DMC5)
VYGIDHYIGGTFSRGSGTRSGPVFNPSTGEEIYRCNYADRATVDEVAKVAVAAGRAWAAQ
SQTRRLQVIFKMRELVIANIEALAEITGLEHGKTIDDAKGEIGRAVEALEFATNAPQVTK
GEYSVNVGGGIDTFSIRQPVGVVGCIAPFNFPVMVPLMMGAMAVAVGNAVVVKPSERVPG
ATALVSELWKKAGLPDGVWNVVNGDAEVVNAILEHPSIAAISFVGSTGVGEYIYQKGCSH
NKRVAAYTGGKNHMVVMPDADLEAAAAAFISAGYGSASQRCMAISLLVAVGDQTADKLRA
LIVPMVEKLRVGSYNDKSADFGPLVTAAAKATVEAAIDRAVAEGAELVVDGRVSVEGRNG
FYVGASLVDNVTTDMQMWKEEIFGPVRGMMRVASLDKAIEVVNAHEYGNGAVIFTRNGRA
AQRFYMEVEAGMQGVNVPVPVPVGHHNFGGLRRSKFGDAHMFGPDAARFYTKMKTISQRW
PEPDAALDHAVSMSFPSNG