Protein Info for GFF2769 in Xanthobacter sp. DMC5

Annotation: Fumarate reductase flavoprotein subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 480 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01266: DAO" amino acids 7 to 209 (203 residues), 54.2 bits, see alignment E=6.1e-18 PF12831: FAD_oxidored" amino acids 7 to 72 (66 residues), 30.3 bits, see alignment E=9.9e-11 PF00890: FAD_binding_2" amino acids 7 to 459 (453 residues), 165.8 bits, see alignment E=6.8e-52

Best Hits

KEGG orthology group: None (inferred from 54% identity to bxe:Bxe_B1003)

Predicted SEED Role

"Fumarate reductase flavoprotein subunit (EC 1.3.99.1)" in subsystem Succinate dehydrogenase (EC 1.3.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.1

Use Curated BLAST to search for 1.3.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (480 amino acids)

>GFF2769 Fumarate reductase flavoprotein subunit (Xanthobacter sp. DMC5)
MIEHAYDVVVVGCGIAGLSAAVSAAETGRRVAVLERSCIEERGGNTRWTEAFMRMKNENE
VSDDFEEHFMQNAGWNLDPSLIAETGRPYADWSPIVKALSFTDPEVISTLAREAGPTLSW
LRGAGIKFSDMATYLITSSTTRICPVGGGLALVEALAAAAEKLGVDFHYETSARGLVRAE
DGSVAGIMAQTGDGMRRFLGKVVLACGGFEGNSEMQARYYGAQARYIRPVARGGYYNRGE
GIKMALEAGAAPCGDYTEYHAEPIDPRSGQSEPIVFVFPYGILVDRDGQRFVDEASGTVD
TIYENIARLIGRLPGGTAYVICDARLDDVPNWQRTVRSDKKPFTADTLEELALQLDIPPQ
KLAATVAAFNAATRPGTFKPLECDGLATEGISPPKSNWALPLAQGPFRAWPISSANCFTF
GGLRCNANAQVVDHDGQPIANLYAAGETMGIYYGRYTGATSVLRGAVFGRIAGRHAVGAN