Protein Info for PS417_14060 in Pseudomonas simiae WCS417

Annotation: glutathione reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 TIGR01424: glutathione-disulfide reductase" amino acids 3 to 447 (445 residues), 621.7 bits, see alignment E=3e-191 PF07992: Pyr_redox_2" amino acids 6 to 317 (312 residues), 252 bits, see alignment E=3.1e-78 PF13738: Pyr_redox_3" amino acids 119 to 291 (173 residues), 56.1 bits, see alignment E=1.3e-18 PF00070: Pyr_redox" amino acids 168 to 244 (77 residues), 70.6 bits, see alignment E=4.6e-23 PF02852: Pyr_redox_dim" amino acids 338 to 446 (109 residues), 127.9 bits, see alignment E=6.9e-41

Best Hits

Swiss-Prot: 83% identical to GSHR_PSEAE: Glutathione reductase (gor) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00383, glutathione reductase (NADPH) [EC: 1.8.1.7] (inferred from 97% identity to pfs:PFLU2986)

MetaCyc: 46% identical to glutathione reductase (NADPH) (Escherichia coli K-12 substr. MG1655)
Glutathione-disulfide reductase. [EC: 1.8.1.7]

Predicted SEED Role

"Glutathione reductase (EC 1.8.1.7)" in subsystem Glutathione: Redox cycle (EC 1.8.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UC90 at UniProt or InterPro

Protein Sequence (452 amino acids)

>PS417_14060 glutathione reductase (Pseudomonas simiae WCS417)
MAYDFDLYVIGAGSGGVRAARFAAGFGAKVAVAESRYLGGTCVNVGCVPKKLLVYGAHFA
EDFEQASGFGWSLGEANFDWATLIANKDREINRLNGIYRNLLVNSGVTLHEGHARLVDAH
QVEINGERFTAKHVLIATGGWPQIPEIPGHEHAIGSNEAFFLKELPKRVLVVGGGYIAVE
FAGIFHGLGAQTTLLYRGDLFLRGFDGSVRTHLKEELTKRGLDLQFNADIERIDKQADGS
LKATLKDGRVLEADCVFYATGRRPMLDNLGLENTGVKLDKRGFIEVDDQYQTADASILAI
GDVIGRVQLTPVALAEGMAVARRLFKPEQYRPVDYAMIPTAVFSLPNIGTVGLTEEEAKE
KGHKVQIFESRFRPMKLTLTECQEKTLMKLVVDADTDQVLGCHMVGPDAGEIVQGLAIAL
KAGATKQHFDDTIGVHPTAAEEFVTMRTPVAD