Protein Info for GFF2755 in Xanthobacter sp. DMC5

Annotation: Soluble epoxide hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12146: Hydrolase_4
  • amino acids 25 to 120 (96 residues), 35.2 bits, E=1.7e-12
• PF00561: Abhydrolase_1
  • amino acids 26 to 127 (102 residues), 95.9 bits, E=6.5e-31
• PF12697: Abhydrolase_6
  • amino acids 27 to 276 (250 residues), 85.9 bits, E=1.3e-27
• PF03096: Ndr
  • amino acids 76 to 284 (209 residues), 31.8 bits, E=1.3e-11

Best Hits

Swiss-Prot: 37% identical to DEH1_MORSB: Haloacetate dehalogenase H-1 (dehH1) from Moraxella sp. (strain B)

KEGG orthology group: None (inferred from 83% identity to bid:Bind_0971)

MetaCyc: 37% identical to fluoroacetate dehalogenase monomer (Moraxella sp. B)
Haloacetate dehalogenase. [EC: 3.8.1.3]

Predicted SEED Role

"Alpha/beta hydrolase fold (EC 3.8.1.5)" (EC 3.8.1.5)

MetaCyc Pathways

• fluoroacetate degradation (1/1 steps found)
• 1,2-dichloroethane degradation (3/4 steps found)
• γ-hexachlorocyclohexane degradation (6/11 steps found)
• butachlor degradation (1/6 steps found)

KEGG Metabolic Maps

• 1,2-Dichloroethane degradation
• 3-Chloroacrylic acid degradation
• Naphthalene and anthracene degradation
• gamma-Hexachlorocyclohexane degradation

Isozymes

Compare fitness of predicted isozymes for: 3.8.1.3, 3.8.1.5

Use Curated BLAST to search for 3.8.1.3 or 3.8.1.5

Protein Sequence (287 amino acids)

>GFF2755 Soluble epoxide hydrolase (Xanthobacter sp. DMC5)
MTEIRHHTVRANGIRQHYLEAGAGAPVVLLHGFPETSFAWRHQIPALAAHYRVIAPDLRG
YGETDKPAAGYDKRTMANDLVALLESLDMEKIALVGHDRGARVATRFAKDHPERLDRLVV
MDNVPTRIVAGSMNAQIAKAYWFFMFHLVPDLPEALIAGREDLWLRHFFSDWCFNPHTIS
GADFDTYVAAYRAPGAVRGAMADYRANAEDNAQDLVDAEVKIGCPVLSLWGADFGAVGKS
FDMAQVWAEMAENLVAVPIERCGHLPHKEQPAVVNRLLLDFLDGWRG