Protein Info for GFF2740 in Sphingobium sp. HT1-2

Annotation: Diaminobutyrate--2-oxoglutarate transaminase (EC 2.6.1.76)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02407: diaminobutyrate--2-oxoglutarate aminotransferase
  • amino acids 18 to 424 (407 residues), 600.2 bits, E=1.6e-184
• TIGR00709: 2,4-diaminobutyrate 4-transaminase
  • amino acids 19 to 429 (411 residues), 441.2 bits, E=3.5e-136
• PF00202: Aminotran_3
  • amino acids 32 to 421 (390 residues), 258.9 bits, E=3.6e-81

Best Hits

Swiss-Prot: 58% identical to ECTB_CHRSD: Diaminobutyrate--2-oxoglutarate transaminase (ectB) from Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)

KEGG orthology group: K00836, diaminobutyrate-2-oxoglutarate transaminase [EC: 2.6.1.76] (inferred from 81% identity to sal:Sala_2950)

MetaCyc: 58% identical to diaminobutyrate-2-oxoglutarate transaminase monomer (Halomonas elongata DSM 2581)
Diaminobutyrate--2-oxoglutarate transaminase. [EC: 2.6.1.76]

Predicted SEED Role

"Diaminobutyrate-pyruvate aminotransferase (EC 2.6.1.46)" in subsystem Ectoine biosynthesis and regulation (EC 2.6.1.46)

MetaCyc Pathways

• ectoine biosynthesis (5/5 steps found)
• norspermidine biosynthesis (3/6 steps found)
• superpathway of polyamine biosynthesis III (4/8 steps found)
• ectoine degradation (1/4 steps found)
• rhizobactin 1021 biosynthesis (1/7 steps found)
• pyoverdine I biosynthesis (3/11 steps found)
• baumannoferrin biosynthesis (1/11 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.46 or 2.6.1.76

Protein Sequence (436 amino acids)

>GFF2740 Diaminobutyrate--2-oxoglutarate transaminase (EC 2.6.1.76) (Sphingobium sp. HT1-2)
MTVTTQPKPTSAMPDTAIYERRESAVRSYARAMPRQFNRAQGVWMHDNQGGRYLDFLSGC
STLNYGHNHPVLKAALVDYIAGDGITHGLDLHTDAKADFLATLEDVILRPRGLDYRAMFT
GPTGTNAVEAAIKLARKITGRELVIAFTNGFHGMTLGALACTGNAAKRGGAGVPLSHVAH
EPYDGYHGPDVDTADLLERRLSDPSSGLDAPAAILVETVQGEGGLNAASPQWLRRIAEIA
RRHGALMIVDDIQAGCGRTGNFFSFEGMGFTPDIVTMAKSLSGMGLPFALTLFRPELDQW
APGEHNGTFRGNNHAFVTATAALRHFWGDAQFEQDIARRGALLERQLDAMAAEHGLSTRG
RGMMRGIDVGSGEVAQTITAACFAQGLIIETSGAHDEIVKILAPLVIEDAVLSAGLDILE
ESIRSALTVTYGVAAE