Protein Info for GFF2735 in Sphingobium sp. HT1-2

Annotation: Uncharacterized MFS-type transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 transmembrane" amino acids 61 to 83 (23 residues), see Phobius details amino acids 95 to 116 (22 residues), see Phobius details amino acids 121 to 143 (23 residues), see Phobius details amino acids 163 to 186 (24 residues), see Phobius details amino acids 195 to 214 (20 residues), see Phobius details amino acids 248 to 266 (19 residues), see Phobius details amino acids 285 to 303 (19 residues), see Phobius details amino acids 315 to 334 (20 residues), see Phobius details amino acids 338 to 365 (28 residues), see Phobius details amino acids 375 to 399 (25 residues), see Phobius details amino acids 406 to 425 (20 residues), see Phobius details PF00083: Sugar_tr" amino acids 28 to 224 (197 residues), 85.3 bits, see alignment E=8.7e-28 amino acids 234 to 434 (201 residues), 59.3 bits, see alignment E=6.7e-20 PF07690: MFS_1" amino acids 68 to 383 (316 residues), 118.2 bits, see alignment E=8.2e-38 PF13347: MFS_2" amino acids 165 to 363 (199 residues), 35.9 bits, see alignment E=7e-13 PF01306: LacY_symp" amino acids 264 to 430 (167 residues), 26.3 bits, see alignment E=6.7e-10

Best Hits

KEGG orthology group: K03762, MFS transporter, MHS family, proline/betaine transporter (inferred from 79% identity to sal:Sala_2955)

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (444 amino acids)

>GFF2735 Uncharacterized MFS-type transporter (Sphingobium sp. HT1-2)
MTASAVPATPAAPVQADKATLRRAIAASALGNATEWFDYGIYAYGVTYISAALFPGEVDD
AILFALATFAISFLVRPLGGLFWGPLGDRLGRKSVLALTILLMSGATLAVGLIPAYAQIG
WAAPALLIILRMVQGFSTGGEYGGAATFMAEYAPDARRGFYGSFLEFGTLAGFSFGAALM
LGFSLLLGDAVMHAWGWRIPFLVAAPLGLIGMYLRSRMEDTPVFREQAAEGNGSPSLSRL
LRDHKRPMLVVGGLVVALNVVNYTLLSYMPTYLQRRIGLSPDEALVVPIIGMLFMMLFLP
IAGHMSDRIGRRPMWRLSLIGLLVAVVPLYMLMATGLVGAIIGFVLLGLLYVPQLATISA
TFPALFPTIARFAGFAIAYNLSTSLFGGTAPAIGSALIGATGNELMPAFYMMAACIIGLI
ALRFMPETAGRSLHDDPFGRERAA