Protein Info for GFF2734 in Variovorax sp. SCN45

Annotation: DNA topoisomerase IV subunit B (EC 5.99.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 665 PF02518: HATPase_c" amino acids 46 to 187 (142 residues), 50.7 bits, see alignment E=4.6e-17 PF00204: DNA_gyraseB" amino acids 235 to 407 (173 residues), 119 bits, see alignment E=3.2e-38 PF01751: Toprim" amino acids 435 to 549 (115 residues), 45 bits, see alignment E=2e-15 PF00986: DNA_gyraseB_C" amino acids 592 to 655 (64 residues), 73.3 bits, see alignment E=2.9e-24

Best Hits

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 97% identity to vap:Vapar_3428)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (665 amino acids)

>GFF2734 DNA topoisomerase IV subunit B (EC 5.99.1.3) (Variovorax sp. SCN45)
MATPPKTPPNSSSASSGYSEGSIRVLKGLEPVKQRPGMYTRTDNPLHIIQEVIDNAADEA
LAGHGKKIKVTLHADGSVSIEDDGRGIPFGLHPEEKAPVVELVYTRLHAGGKFDKGSGGA
YSFSGGLHGVGVSVTNALSKRLEVSTHREGSIAKLAFSGGDVIEQLEVRKLEAGERKQGT
TVRAWPDAKYFETAALPMGELTHLLRSKAVLMPGVSVTLTNEKTKESQQWLYKGGLSDYL
MQTLNGDPVIPLFEGGGHADKNADNFAEGEGADWCVAFTEDGQPVRESYVNLIPTSAGGT
HESGLRDGLFTAVKGFIELHSLLPKGVKLLPEDVFARASYVLSAKVLDPQFQGQIKERLN
SRDAVRLVSSFVRPALELWLNQHVDYGKKLAELAIKAAQTRQRAGQKVEKRKGSGVAVLP
GKLTDCESKDISHNEVFLVEGDSAGGSAKMGRDKESQAILPLRGKVLNTWEVERDRLFAN
TEIHDISVAVGVDPHGPNDTPDMSGLRYGKICILSDADVDGSHIQVLLLTLFFRHFPKLI
EAGHVYVAKPPLFRVDAPARGKKPASKVYALDEGELTATLDKLRKDGVREGAWSISRFKG
LGEMSAEQLWETTLNPDTRRLMQVQLGRFDFTSTQGEITKLMGKGEAAARRELMELRADD
VDIDV