Protein Info for GFF2730 in Variovorax sp. SCN45

Annotation: DNA topoisomerase IV subunit A (EC 5.99.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 801 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 19 to 793 (775 residues), 684.1 bits, see alignment E=1.1e-209 PF00521: DNA_topoisoIV" amino acids 40 to 506 (467 residues), 420 bits, see alignment E=5.6e-130

Best Hits

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 97% identity to vpe:Varpa_2187)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (801 amino acids)

>GFF2730 DNA topoisomerase IV subunit A (EC 5.99.1.3) (Variovorax sp. SCN45)
MDSQPPLDLQGPTDGDTTLTLADYAQTAYLEYALSVVKGRALPDVSDGQKPVQRRILYSM
SRMGLGFGGTNGTVGAKPVKSARVVGDVLGRFHPHSDQAAYDALVRMAQDFSQRYPLVDG
QGNFGSRDGDGAAAMRYTEARLARITSLLLDEIDEGTVDFIPNYDGSTEEPRLLPARLPF
TLLNGASGIAVGLATEIPSHNLREIADACVALIKSNGKLSEEELFAIVPGPDYPGGAQII
SNASDIADAYRTGRGSLKVRARWKIEELARGQWQLVVNELPPGVSTQKVLEEIEEITNPK
VKAGKKALSADQTQLKAAMLSVLDVVRDESSKDAAVRLVFEPKTSRISQDEFITTLLAQT
SLETSSPINLTMVGIDGKPTQKSLRQMLNEWIAFREVTVEKRSRHRLNKVLDRIHILEGR
QLVLLNIDEVIAIIRAAEDPKAALIARFNLSDRQAEDILEIRLRQLARLEAIKIEQELSG
LRDEQKKLEDILGSPAALRRLLVKEIEADAKTFEDPRRTLIQAEKRAVAEVKVVDEPVTV
IVSQKGWVRAQKGWASEKAAAANGGNGASAPEYSFKSGDSLYGAFECRSVDTLLVFGSAK
DKSVRVYTVPVASLPGARGDGQPVTTLIELDAGTHVTHFFAGPVGASVLLANTGGYGFIA
TVENMMSRQRGGKAFIDVGEGEQLCRPSLVGGASGAEPMPAATHVACASTGGRILTFDIT
ELKSLPKGGRGLTLIDLEPKDTLAGAAAYTRSVKIEGIGRGAKEREETLEIRTLNNARGS
RARKGKAADLGFKPSSIVRVQ