Protein Info for GFF2726 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1002 PF14850: Pro_dh-DNA_bdg" amino acids 68 to 170 (103 residues), 89.3 bits, see alignment E=3.7e-29 PF01619: Pro_dh" amino acids 184 to 478 (295 residues), 331 bits, see alignment E=1.1e-102 TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase" amino acids 540 to 982 (443 residues), 578 bits, see alignment E=7.3e-178 PF00171: Aldedh" amino acids 542 to 984 (443 residues), 358 bits, see alignment E=1e-110

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 76% identity to vap:Vapar_4810)

Predicted SEED Role

"Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.12 or 1.5.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1002 amino acids)

>GFF2726 Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VNSPIHRFLEPSMPRAQDRLPFPYRPEAGVLAQRLGALKGALDWAAAAGDAAPWVRAVRE
NPPPFWAMESLLKEYPISSAEGLALMRLAEALLRVPDAETAIALTADQLGRADFDGAADS
TLARLSSTAIAMSKHFLPSVGSEPGLMAKLGARTVVAATLRAVQLLGRQFVLGQTIVEGM
KEAAAARKRQANLRFSFDMLGEGARTDEDALRYLESYRNAIAAIARSADPARHPSQNDGI
SIKLSALHPRYEDAQHDRVMRELVPRVWSLCEAAAAAHLNLTIDAEEVDRLELSLAVFEA
LATRVAQHCPQWQGFGLALQAYQTRAVELIEHVADLARQHRLKFMCRLVKGAYWDAEIKR
AQELGLPAYPVFTQKPHTDVSYLACARALLAAHDAIHPQFATHNAGTIAAILQMAGKQGT
PFELQRLHGMGEGIYREVLKNPAVSCRVYAPVGAHRDLLAYLVRRLLENGANSSFVHQLA
DESVGMDELLVSPLAQSRPEAVASSLPLPPDLYGPARPNSQGLDLAVAAHREPLLAAHAA
LVVPAVAESRAADAPAAVEAAVRAFAGWNHAPLTERAAVLRRAADALQQQMPRLCALLVK
EAHKGWGDAVSEVREAIDFLRYYANDAERVMAPQTLPGPTGESNTLRLEGRGAWVCISPW
NFPLAIFIGQVAAALVTGNTVLAKPAEQTPAIALEAVKLLHAAGVPAEALQLLHGPGDTV
GAALVAQAGVAGVVFTGSTQVAKLIQRALAAKDGPLVPLIAETGGINAMLVDSTALPEQV
ADAVVQSAFRSAGQRCSALRLLCVHEAVADGVIAMIGGAASELVAGDPSALSTDLGPVID
AEAFEGIARHLQRLRQQARPLLEARPSTDLPQLVAPQMFEVGDIAEVGQEIFGPVLQVVR
WSGDPMAVIQRINALGYGLTLGIQTRIDSRALALAGAARVGNVYVNRNMIGAVVGVQPFG
GEGLSGTGPKAGGPHYLQRFCAEQTLTINTTAAGGNVALFTG