Protein Info for PS417_13815 in Pseudomonas simiae WCS417

Annotation: 4-amino-4-deoxy-L-arabinose-phospho-UDP flippase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 133 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 46 to 67 (22 residues), see Phobius details amino acids 77 to 97 (21 residues), see Phobius details amino acids 103 to 120 (18 residues), see Phobius details PF00892: EamA" amino acids 33 to 120 (88 residues), 31.1 bits, see alignment E=1.3e-11

Best Hits

Swiss-Prot: 77% identical to ARNF_PSEF5: Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K12963, undecaprenyl phosphate-alpha-L-ara4N flippase subunit ArnF (inferred from 90% identity to pfs:PFLU3037)

Predicted SEED Role

"Polymyxin resistance protein PmrM" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance )

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U3U5 at UniProt or InterPro

Protein Sequence (133 amino acids)

>PS417_13815 4-amino-4-deoxy-L-arabinose-phospho-UDP flippase (Pseudomonas simiae WCS417)
MSRVQGFALALGSVGLVSAAQLGMRWSMTRLPLPNQWLSVQIDLDALGVVLLAILAYALS
MLCWLGALKHLPLGRAYSLLSISYALVYLLAASLPVFNEDFSISKTLGVALVILGVLVIN
SRRASATSPRNAP