Protein Info for GFF269 in Variovorax sp. SCN45

Annotation: 2-methylaconitate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 transmembrane" amino acids 321 to 332 (12 residues), see Phobius details TIGR02334: probable AcnD-accessory protein PrpF" amino acids 5 to 396 (392 residues), 754.5 bits, see alignment E=1e-231 PF04303: PrpF" amino acids 6 to 392 (387 residues), 575.7 bits, see alignment E=2e-177

Best Hits

Swiss-Prot: 85% identical to PRPF_CUPNE: 2-methyl-aconitate isomerase from Cupriavidus necator

KEGG orthology group: K09788, hypothetical protein (inferred from 87% identity to reh:H16_A1908)

Predicted SEED Role

"2-methylaconitate cis-trans isomerase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (396 amino acids)

>GFF269 2-methylaconitate isomerase (Variovorax sp. SCN45)
MVSAPQIKIPATYMRGGTSKGVFFRLQDLPEAARVPGKARDALLLRVIGSPDPYGKQIDG
MGAATSSTSKTVILSKSERPGHDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPFAISNGL
VDAGRVPRDGVAVVRIWQANIGKTIVAHVPMTGGAVQETGDFELDGVTFAAAEVQLEFMD
PAAEEEGAGGAMFPTGQLVDELEVPGVGTLRATMINAGIPTIFVNAEAIGYTGTELQDAI
NGDPAALARFEAIRAHGAVRMGLIKHIGEAAGRQHTPKVAFVAKPAGYTASSGKQVAAGD
VDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVG
AQASKVDGEWQVTRAVMSRSARVLMEGWVRIPGDAF