Protein Info for PS417_13550 in Pseudomonas simiae WCS417

Annotation: amino acid ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF10613: Lig_chan-Glu_bd" amino acids 30 to 114 (85 residues), 35.5 bits, see alignment E=9.9e-13 PF00497: SBP_bac_3" amino acids 35 to 253 (219 residues), 111.5 bits, see alignment E=2.7e-36

Best Hits

KEGG orthology group: K02030, polar amino acid transport system substrate-binding protein (inferred from 46% identity to pfl:PFL_5021)

Predicted SEED Role

"Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1)" in subsystem Arginine and Ornithine Degradation (TC 3.A.1.3.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U3Q8 at UniProt or InterPro

Protein Sequence (284 amino acids)

>PS417_13550 amino acid ABC transporter substrate-binding protein (Pseudomonas simiae WCS417)
MKSKRMATWLGSAVLMLAAGFSWAAEKPIVFAVAAEPYPPFTVKGGNGQWSGFEVDLIHK
LCEGMKAECQIKEVAWDGIIPSLLAKKIDVIFSSMSVTDEREKQIAFSRAYYDSLLGVAG
PKGSEVEISPAGLKGKLIGVQISTVSANYLKKYYENIADLKYYDTQESANADLIAGRIDY
MMADDTAIAMMVKTPEASGLAHIASVPYDPIIGRGVGAGLRKEDTALKARLDKAIGELLV
SKDYDDLSQHYFGLSVSPCKRTDTPAFVSKTCDSPYPQIAQKTE