Protein Info for GFF2654 in Sphingobium sp. HT1-2

Annotation: Phosphate ABC transporter, permease protein PstA (TC 3.A.1.7.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 transmembrane" amino acids 31 to 52 (22 residues), see Phobius details amino acids 200 to 221 (22 residues), see Phobius details amino acids 241 to 261 (21 residues), see Phobius details amino acids 267 to 287 (21 residues), see Phobius details amino acids 317 to 338 (22 residues), see Phobius details amino acids 350 to 373 (24 residues), see Phobius details amino acids 386 to 406 (21 residues), see Phobius details PF11812: DUF3333" amino acids 16 to 157 (142 residues), 124.7 bits, see alignment E=3.8e-40 TIGR00974: phosphate ABC transporter, permease protein PstA" amino acids 172 to 411 (240 residues), 221.9 bits, see alignment E=4.3e-70 PF00528: BPD_transp_1" amino acids 212 to 408 (197 residues), 64.6 bits, see alignment E=1e-21

Best Hits

KEGG orthology group: K02038, phosphate transport system permease protein (inferred from 90% identity to sjp:SJA_C1-28880)

Predicted SEED Role

"Phosphate transport system permease protein PstA (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (414 amino acids)

>GFF2654 Phosphate ABC transporter, permease protein PstA (TC 3.A.1.7.1) (Sphingobium sp. HT1-2)
MSAERLPTDWKSDAMRKRIARRYAAERRFKAAGMAAVLLSAAFLAFLLFTMVGNGMRGFT
RTEIALKVDFPASPLLLDPANITDEALANANLPMVTSTVAQKALGKDADAMISPSAWIAI
RDAIKADPKILARTETIDVPAATAIDLAAKSKGSPEAEAQVARLRQAGLLATGINWGFLT
ASDGTDPTQVGIWGAFKGSLLTMLVTLVLSFPIGVATALYLEEYARKSWWTDIIEVSINN
LAAVPSIIFGLLGLSIFLNLLHLPRSAALVGGMTLALMTMPVIVIAGRNAIKSVPPSIRD
AALGIGASPVQVVFQHVLPLALPGILTGTIIGMARALGETAPLLMIGMRAFIATPPGGIV
DPATVLPVQIFLWSDEVSKGFVEKTSAAIIVLLAFLLAMNGLAIYLRNKFEKRW