Protein Info for PS417_13485 in Pseudomonas simiae WCS417

Annotation: lipase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 470 PF00353: HemolysinCabind" amino acids 367 to 398 (32 residues), 32.4 bits, see alignment (E = 3.6e-12)

Best Hits

Swiss-Prot: 89% identical to LIPB_PSEFL: Lipase from Pseudomonas fluorescens

KEGG orthology group: None (inferred from 90% identity to pfs:PFLU3141)

Predicted SEED Role

"Lipase (EC 3.1.1.3)" (EC 3.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.3

Use Curated BLAST to search for 3.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UBY2 at UniProt or InterPro

Protein Sequence (470 amino acids)

>PS417_13485 lipase (Pseudomonas simiae WCS417)
MGIFDYKNLGTEGSKALFADAMAITLYSYHNLDNGFAVGYQHNGLGLGLPATLVGALLGS
TDSQGVIPGIPWNPDSEKAALDAVHKAGWTPISASTLGYSGNVDARGTFFGEKTGYTTAQ
VEVLGKYDDAGKLLEIGIGFRGTSGPRETLISDSIGDLVSDLLAALGPKDYAKNYAGEAF
GGLLKNVADYASAHGLSGHDVVVSGHSLGGLAVNSMADLSNSKWSGFYKDANYVAYASPT
QSASDKVLNIGYENDPVFRALDGSSFTLSSLGVHDTPHDSTTDNIVSFNDHYASTLWNVL
PFSIVNLPTWVSHLPTGYGDGLSRVLDSGFYEQMTRDSTVIVANLSDPARATTWVQDLNR
NAEPHKGNTFIIGSDGHDLIQGGKGADFIESGKGNDTIRDNSGHNTFLFSGQFGQDRIIG
YQPSDQLVFKEVEGSAQYREQGGDTVISFGADSVTLVGVNGWSGEGVAIG