Protein Info for PS417_13475 in Pseudomonas simiae WCS417

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 985 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00082: Peptidase_S8" amino acids 65 to 354 (290 residues), 147.7 bits, see alignment E=7.1e-47 TIGR02601: autotransporter-associated beta strand repeat" amino acids 450 to 481 (32 residues), 43.7 bits, see alignment (E = 1.8e-15) PF12951: PATR" amino acids 451 to 480 (30 residues), 41.4 bits, see alignment (E = 1.7e-14) TIGR01414: outer membrane autotransporter barrel domain" amino acids 515 to 985 (471 residues), 134 bits, see alignment E=7.5e-43 PF03797: Autotransporter" amino acids 713 to 965 (253 residues), 198.2 bits, see alignment E=3.1e-62

Best Hits

KEGG orthology group: K12685, subtilase-type serine protease [EC: 3.4.21.-] (inferred from 73% identity to pfo:Pfl01_2683)

Predicted SEED Role

"Serine protease homologue"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UL09 at UniProt or InterPro

Protein Sequence (985 amino acids)

>PS417_13475 membrane protein (Pseudomonas simiae WCS417)
MITDSPRFKPFTATSLLLLSVAAQAAYTESGQPGNAASWRSSEFQSDWGLGRMKADEAYA
AGITGSGVKIGALDSGFDPNHPEASRDRYHAVSASGSYVDGSAFSTTGALNPNNDSHGTH
VTGTMGAARDGVGMHGVAYNAQLYVGNTNANDSFLFGPTPDPKYFKAVYSALVDSGVRAI
NNSWGSQPKDVSYRTLADLHAAYAQHYNQGTWLDAAADVAKAGVINVFSAGNSGYANASV
RSALPYFQPELEGHWLAVSGLDKANNQKYNQCGIAKYWCISTPGALINSTIPDGGYGVKS
GTSMSAPHATGALALVMERYPYMTNEQALQVLLTTATQLDGSLTQAPTTTVGWGVPDLGR
AMHGPGQLLGPMNVSLAAGQGDVWSNGISDQALLRRQAEDAIEHSAWQQTLIDKGWQNGV
GASASQQDQTDYAVGTARDQAAATRIYEGSLIKSGAGTLVLSGDSTYRGATTVNGGLLAV
NGSLTSAVTVNDSGTLGGSGRIAALSVHPGGRVAPGNSVGTLQVAGDVNLGAGSTYAVEL
TPIDSDRIVAGGRAILGGGNVTLALENSPTLLSGAQAQSLIGRQYTILQAVGGIQGQFGQ
VLPDYLFLGGTLAYATDGVQLDVVRNDATFASVAATRNQRNVAAAAEQLGAGNPVYESVL
QSDSIAVAQQGLQQLSGEIYPAIGAMLINDSQSLRDAVGERLRHVPVNAESNLWLKALGA
WGKADSRSETAGSTTSLGGLLGGVEGALDEQTRVGLVAGYSDSSLNMGSGTHSSASIDSY
HLGAYAGRELGDWRVSAGGAYSWHRGDVKRDLHYGAVSGKQNAKLDAHTAQLFTEAAYRI
HLQPLALEPFANLAYVHLDSEAFHEKGAAAALERGSDRRDAVLSTLGMRALKTVALNDHQ
QLQLSGSLGWQHSLTAIESEEHLAFVAGGPSFAVRSSPLLRDAALVGVQASLALSPVTRV
NLDYNGQLGGREKTQGVGLSLNWQF