Protein Info for PS417_13465 in Pseudomonas simiae WCS417

Annotation: hemolysin D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 transmembrane" amino acids 13 to 35 (23 residues), see Phobius details TIGR01843: type I secretion membrane fusion protein, HlyD family" amino acids 14 to 433 (420 residues), 387.5 bits, see alignment E=4.2e-120 PF00529: CusB_dom_1" amino acids 26 to 389 (364 residues), 53.6 bits, see alignment E=4.5e-18 PF13533: Biotin_lipoyl_2" amino acids 57 to 100 (44 residues), 36.5 bits, see alignment 8.1e-13 PF13437: HlyD_3" amino acids 285 to 385 (101 residues), 53.8 bits, see alignment E=7.3e-18

Best Hits

Swiss-Prot: 50% identical to APRE_PSEAE: Alkaline protease secretion protein AprE (aprE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K12537, protease secretion protein HasE (inferred from 95% identity to pfs:PFLU3143)

Predicted SEED Role

"ABC-type protease exporter, membrane fusion protein (MFP) family component PrtE/AprE" in subsystem Protein secretion by ABC-type exporters

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U1P8 at UniProt or InterPro

Protein Sequence (433 amino acids)

>PS417_13465 hemolysin D (Pseudomonas simiae WCS417)
MSSITFEQRDANFFARMGWLLTVVGAGGFFLWASLAPLDQGIPVQGTVVVSGKRKAVQTL
SPGVVSRILVKEGERVKQGQPLFRLDQTQHQADVQSLQAQYRMAWASVARWQSERDNRAS
ILFPPELSANPDPALALVLEGQRQLFSSRREAFAREQAGIRANIDGATAQLNGMRRARSD
LTAQAQSLRDQLSNLQPLADNGYIPRNRLMDYQRQLSQVQQDLAQNTGESGRVEQGILES
RLKLQQHSEEYQKEVRSQLADSQLRSLTLEQQLTSAGFDLQHSEINAPADGIAVNLSVHT
EGAVVRAGETLLEIVPQDTRLEVEGHLPVHLVDKVGTHLPVDILFTAFNQSRTPRVPGEV
SLISADQMLDEKTGAPYYVLRTTVSEAALEKLHGLVIKPGMPAEMFVRTGERSLLNYLFK
PLLDRAGSALTEE