Protein Info for GFF2638 in Xanthobacter sp. DMC5

Annotation: Type IV secretion system protein virB4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 809 PF03135: CagE_TrbE_VirB" amino acids 182 to 388 (207 residues), 21.1 bits, see alignment E=2.6e-08 PF19044: P-loop_TraG" amino acids 576 to 726 (151 residues), 34.2 bits, see alignment E=1.2e-12

Best Hits

KEGG orthology group: K03199, type IV secretion system protein VirB4 (inferred from 85% identity to mpo:Mpop_3089)

Predicted SEED Role

"Conjugative transfer protein TrbE" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (809 amino acids)

>GFF2638 Type IV secretion system protein virB4 (Xanthobacter sp. DMC5)
MMNLAEYRRQGSRLADYLPWAALVGSGIVLNKDGSFQRTARFRGPDLDSAVPAELVAVAG
RLNSAFRRLGSGWALFVEAQRHEAGAYPESAFPDPASALVEAERKAGFAEAGSHFESSYF
LTFCWLPPAEDAARAESWLYEGRGREGVDPREAVSAFIDRTDRVLRLVEAFMPECRWLDD
AETLTYLHGCVSTRRQRLRVPETPMYLDALLADQPLTGGLEPMLGAEHLRVLTIVGFPTA
TTPGILDDLNRLAFPYRWSTRAILLDKTDATRLLTKIRRQWFAKRKSIAAILKEVMTNEA
SVLVDSDAANKADDADLALQELGADHAGIAYVTATVTVWDADPRVANEKLRLVEKVIQGR
DFTAMVETINAVDAWLGSLPGHVYANVRQPPVSTLNLAHMIPLSAVWAGDEQDGHFAAPP
LFYGKTEGSTPFRFSLHVGDVGHTLVVGPTGAGKSVLLALMALQFRRYAGSQVFAFDFGG
SIRAAVLAMGGDWHDLGGGLAEDSTALQPLSRVDELAERTWAADWIVAILTREGVPITPE
VKERIWSALTSLASAPVEERTLTGLAVLLQSNALKQALRPYCIGGAHGRLLDAEAEHLGQ
ASVQAFETEGLIGTSAAPAVLSYLFHRIEDRLDGSPTLLIIDEGWLALDDPGFAGQLREW
LKTLRKKNASVVFASQSLSDIDGSAIAPAIIESCQTRLLLPNERAVEPQITAIYRRFGLN
DRQIETLARATPKRDYYCQSRRGNRLFELGLSQVALALCATSSKTDQAALSQVLAEHGRD
DFLAAWLRHRGLGWAADLIPTLGNLETRS