Protein Info for GFF2614 in Sphingobium sp. HT1-2

Annotation: Heat-inducible transcription repressor HrcA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 TIGR00331: heat-inducible transcription repressor HrcA" amino acids 12 to 345 (334 residues), 283 bits, see alignment E=2.2e-88 PF01628: HrcA" amino acids 110 to 332 (223 residues), 193.5 bits, see alignment E=2.5e-61

Best Hits

Swiss-Prot: 71% identical to HRCA_SPHAL: Heat-inducible transcription repressor HrcA (hrcA) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K03705, heat-inducible transcriptional repressor (inferred from 90% identity to sjp:SJA_C1-10680)

Predicted SEED Role

"Heat-inducible transcription repressor HrcA" in subsystem Heat shock dnaK gene cluster extended

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (347 amino acids)

>GFF2614 Heat-inducible transcription repressor HrcA (Sphingobium sp. HT1-2)
MAATASIAELNDRARDVFRMVVDSYLGTGLPVGSRTISKMGLSLSAASIRNVMQDLEELG
LLAAPHTSAGRMPTETGLRLFVDGMMQAAEPSVEERRAIEAGLAEGGPIEEALSAATATL
SGLSACAGIVMVPRREPVLRQFGFVPLNDRQALAVLVGQDGSIENRVLDLPRGVTPSMLN
EAANFMSARFGGLTLRQAGDLLAREMEQERSTLEGAARELVTRGIAIWSHDADDRPVLIV
RGQAHLIDDGTAADLDRVRQLLEQLEGKQEISRLLESALAGSATKIFIGSENKLFSLSGS
SVIAAPYRGGDGRVVGVVGVIGPTRLNYARVIPMVDFTAQTLSRLMR