Protein Info for GFF2605 in Variovorax sp. SCN45

Annotation: Tricarboxylate transport sensor protein TctE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 signal peptide" amino acids 1 to 47 (47 residues), see Phobius details transmembrane" amino acids 182 to 202 (21 residues), see Phobius details PF08521: 2CSK_N" amino acids 27 to 172 (146 residues), 127.4 bits, see alignment E=6.8e-41 PF00512: HisKA" amino acids 250 to 313 (64 residues), 27.6 bits, see alignment E=3.8e-10 PF02518: HATPase_c" amino acids 360 to 467 (108 residues), 71.2 bits, see alignment E=1.5e-23

Best Hits

KEGG orthology group: K07649, two-component system, OmpR family, sensor histidine kinase TctE [EC: 2.7.13.3] (inferred from 93% identity to vpe:Varpa_2286)

Predicted SEED Role

"Tricarboxylate transport sensor protein TctE"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (471 amino acids)

>GFF2605 Tricarboxylate transport sensor protein TctE (Variovorax sp. SCN45)
MPSLRLNARLRRASLWQRLALLLFPALLVVTGLELWMTRHDALAAANAAYDRSLLGALKS
IDANISTASGGLSVELPYTMFEFFELTASGQVFFRVSTSDGLVELGSADLPAPPAELKMG
TPAFYDATYFGEPVRLAAYRRDLDRAPAGSTGRSVLVQVGESTRSRQEFTARLVRSAALR
DGLVLAMLLCGTAVALAAALRPLSRLAREVQARTPEDLTRIAEADLPAEVRPLVAAVNQQ
MTRTQDLVTQQRQFLDDASHQLRTHLTTLQMQADYARREQDPAQIQLALDALGTEIGRAT
RSAQQLLALGRSDTVSVEPAGFDMAALLREVAVDLLPLARAKRIDLGIHSPTPEFIAIAD
RGLMREALSNLVTNAIAYTPAEGTITLFAAGDAAGWSLNVEDDGPGLSDEERAALGQRFR
RGARAGKGGFGLGLAIARSIAQRHQGELRLESREAEPGLHAIIWWPRPAAH