Protein Info for GFF2600 in Sphingobium sp. HT1-2

Annotation: Uncharacterized aldehyde oxidase, molybdopterin-binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF20256: MoCoBD_2" amino acids 41 to 168 (128 residues), 62.2 bits, see alignment E=5.9e-21 amino acids 575 to 676 (102 residues), 37.1 bits, see alignment E=2.6e-13 PF02738: MoCoBD_1" amino acids 315 to 531 (217 residues), 123.6 bits, see alignment E=7.6e-40

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 60% identity to nar:Saro_1702)

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (727 amino acids)

>GFF2600 Uncharacterized aldehyde oxidase, molybdopterin-binding subunit (Sphingobium sp. HT1-2)
MNAPILSRRRFLSASLVAGGGLLFDLNISLAEAADGAPHILTAFVRILPDNRVIIGAKNA
EIGQGAKTMLPMLIAEELDVDWAQVTIEQTHADQKIFGGQTAGGSRTTPREWLPTRKAGA
AARAMLVAAAAQQWGVAPATLKTGSGKVSDPASGRSMTYAALAAAAAQQPAPDPATLTLK
DPKDFHIIGQSIGGVDTPAIVAGKPLFGIDFKLPGMLYAVLETCPAFGGTFQSANLDAVK
ALPGVTHVLTIKGDGTPESLFDGVAILSTSWWSANQAREALKVEWDMGAVSGFSTEGYAA
QAAERLKGKADGDIVRAGDVDAAFAGAAKTVSAQYDYPFLAHGTLEPQNCTALFKDGAIE
IWAPTQNPESGRGLVAKALNLPPEKIRINFTRIGGGFGRRLMNDYMVQAAAIAAQLPGVP
VKLLYNRQQDIQRDFYRPAGWHGFRAALDKGGKLTAFHDHFVTFGKDGKPVRSAEMPETE
IPAGLIDTVLLEQSFLATNMPTGWLRAPGSNALAFVTQAFLDEVAQAAGKDLPTLMLELL
GDPRELPRGPNAPPFVTRRARGVIEKVVAMSGWADRGKLPKGRGKGFAFYYSHMGYFAEV
LEVALVDGMPKVATVWVAGDVGSQIINPMNALHQAQGSVIEGLGQALAGQKITQVAGAVE
QANFDTHPLQRIPDTPQIIVEFVKTDYPPTGMGEPALPPVIPALVNALHAATGKRIRTLP
IVPEMFA