Protein Info for PS417_13215 in Pseudomonas simiae WCS417
Annotation: Triostin synthetase I
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 52% identical to DHBE_BACSU: 2,3-dihydroxybenzoate-AMP ligase (dhbE) from Bacillus subtilis (strain 168)
KEGG orthology group: K02312, 2,3-dihydroxybenzoate-AMP ligase [EC: 2.7.7.58] (inferred from 55% identity to tfu:Tfu_1871)MetaCyc: 56% identical to 2,3-dihydroxybenzoate--[aryl-carrier protein] ligase MxcE (Stigmatella aurantiaca Sg a15)
RXN-23311 [EC: 6.2.1.71]
Predicted SEED Role
"2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58)" in subsystem Iron acquisition in Vibrio (EC 2.7.7.58)
MetaCyc Pathways
- superpathway of chorismate metabolism (44/59 steps found)
- enterobactin biosynthesis (5/11 steps found)
- vanchrobactin biosynthesis (2/8 steps found)
- acinetobactin biosynthesis (2/9 steps found)
- vibriobactin biosynthesis (2/9 steps found)
- bacillibactin biosynthesis (4/12 steps found)
- anguibactin biosynthesis (1/8 steps found)
- myxochelin A and B biosynthesis (1/9 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.7.7.58 or 6.2.1.71
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7U1K5 at UniProt or InterPro
Protein Sequence (532 amino acids)
>PS417_13215 Triostin synthetase I (Pseudomonas simiae WCS417) MLDGCTDWPDDFIHRYREQGYWRGAPLGELLREQARRTPHKEALVDGTRRWTYADLNHQA DCLAAGLAERGLVAGQRVLVQLPNVAEFFSLTFALLRLGVIPVFALPAHREHELAHLAHL SQAVAYVIVDQHLGFDYRPLARTLRAQVPSLQQVWVVGAAEEFVPLAQCVASPREWSQPD SRGVAVLLLSGGTTGLPKLIPRTHDDYACNARLAAQACGFDADTRYLAALPVAHNFPLAS PGALGVFSVGATLVLAPEPSPDTAFELIERERITHTALIPPLVLLWLEVAQWSDNDLSSL QWLQVGGARLKAEIAARIRPALGCGLQQVYGMAEGLLCFTPLDDPQALVFDTQGLPLTAD DEIRIVDADDQPVAPGAIGELLVRGPYTIRGYYNAAEQNLRAFTADGFYRSGDLVRRLPE GHLIVEGRSKDVINRGGEKIPVEEIENLLLGHPLIRDVALVALADELLGERSCACVLAHG EQVDLASINAWLRERGLAAYKLPDRLQVLREFPLTRLGKVNRKALAEQVLAL