Protein Info for GFF2570 in Xanthobacter sp. DMC5

Annotation: Protein translocase subunit SecA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 926 PF07517: SecA_DEAD" amino acids 7 to 390 (384 residues), 432.7 bits, see alignment E=2e-133 TIGR00963: preprotein translocase, SecA subunit" amino acids 28 to 827 (800 residues), 1071 bits, see alignment E=0 PF01043: SecA_PP_bind" amino acids 233 to 346 (114 residues), 149 bits, see alignment E=2.1e-47 PF21090: P-loop_SecA" amino acids 405 to 627 (223 residues), 287.4 bits, see alignment E=1.8e-89 PF07516: SecA_SW" amino acids 629 to 841 (213 residues), 223.4 bits, see alignment E=9.3e-70 PF02810: SEC-C" amino acids 906 to 924 (19 residues), 38.7 bits, see alignment (E = 2.1e-13)

Best Hits

Swiss-Prot: 82% identical to SECA_AZOC5: Protein translocase subunit SecA (secA) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K03070, preprotein translocase subunit SecA (inferred from 82% identity to azc:AZC_3307)

Predicted SEED Role

"Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (926 amino acids)

>GFF2570 Protein translocase subunit SecA (Xanthobacter sp. DMC5)
MFGGLARKLFGSANDRRVKGYRPRVQAINALEKELEALSDDQLRARTEDFRRQLAEGQVT
LDDLLVPAFATVREGAKRALGMRHFDVQLIGGMVLHESSIAEMKTGEGKTLVATLPVYLN
ALAGKGVHVVTVNDYLAKRDAEWMGKLYRFLGLTTGIIVHGLDDDERRAAYASDVTYATN
NELGFDYLRDNMKYERAHMVQRPHFYAIVDEVDSILIDEARTPLIISGPLDDRSDFYNTI
DQYIPRLSKEDYEVDEKQRTVSMTEAGMEKMEQMLTEAGLLKSESLYDIENVSVVHHVNQ
ALRAHSLFQRDKDYIVRNDEVVIIDEFTGRMMPGRRYSEGLHQALEAKERVQVQPENQTL
ASITFQNYFRLYEKLAGMTGTAATEAAEFQDIYKLEVVEVPTNVPVSRIDDDDEVYRSAG
EKYAAIIELIKECSARGQPVLVGTTSIEKSELLAELLKQGGFHQIDFSDPAAFAHDRSGK
AFAVLNARYHEQEAYIVSQAGVPGAITIATNMAGRGTDIKLGGAADMRIAAELKDMPEGE
QRTAAETRIRAEVEELKQKALGAGGLFVLGTERHESRRIDNQLRGRSGRQGDPGHSKFFL
SLDDDLMRIFGSDRLDGMLQRLGLKEGEAIIHPWINKALEKAQQKVEARNYDMRKNVLKY
DDVLNDQRKVVFEQRLELMNDDDVAETVEDMRHALITETVAKFIPENAYPEQWDVDGLDA
AIKEVLGIELPVKDWAKEEGIAGPEVTERVIRRADEWMAAKTAQYGPDIMRYVEKSILLQ
TLDHLWREHIAMLDHLRQVVGLRGYAQRDPLNEYKSEAFNLFSALLNRLREVVTAQLMRV
EIVAQPPMEEELPTMEAHHADPFTGEDEMSFAGAALGSRPEPLLSGDVAVIERNPNDPST
WGKVGRNEACPCGSGKKYKHCHGRFA