Protein Info for GFF2569 in Variovorax sp. SCN45

Annotation: Phosphoglycerate mutase (EC 5.4.2.11)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01258: phosphoglycerate mutase 1 family
  • amino acids 2 to 246 (245 residues), 388.6 bits, E=5.5e-121
• PF00300: His_Phos_1
  • amino acids 4 to 126 (123 residues), 105.7 bits, E=1.3e-34

Best Hits

Swiss-Prot: 97% identical to GPMA_VARPS: 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01834, phosphoglycerate mutase [EC: 5.4.2.1] (inferred from 97% identity to vap:Vapar_4103)

MetaCyc: 64% identical to 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (Escherichia coli K-12 substr. MG1655)
RXN-15513 [EC: 5.4.2.11]

Predicted SEED Role

"Phosphoglycerate mutase (EC 5.4.2.1)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes (EC 5.4.2.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (24/26 steps found)
• Bifidobacterium shunt (15/15 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (16/17 steps found)
• homolactic fermentation (12/12 steps found)
• superpathway of anaerobic sucrose degradation (17/19 steps found)
• glycolysis II (from fructose 6-phosphate) (11/11 steps found)
• glycolysis III (from glucose) (11/11 steps found)
• superpathway of glucose and xylose degradation (15/17 steps found)
• glycolysis I (from glucose 6-phosphate) (12/13 steps found)
• Entner-Doudoroff pathway I (9/9 steps found)
• heterolactic fermentation (15/18 steps found)
• Rubisco shunt (9/10 steps found)
• gluconeogenesis I (11/13 steps found)
• gluconeogenesis III (10/12 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• glycolysis IV (8/10 steps found)
• glycolysis VI (from fructose) (8/11 steps found)
• superpathway of hexitol degradation (bacteria) (13/18 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• formaldehyde assimilation I (serine pathway) (9/13 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (6/9 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
• photorespiration II (6/10 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)
• glycerol degradation to butanol (9/16 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Glycolysis / Gluconeogenesis

Isozymes

Compare fitness of predicted isozymes for: 5.4.2.1

Use Curated BLAST to search for 5.4.2.1 or 5.4.2.11

Protein Sequence (247 amino acids)

>GFF2569 Phosphoglycerate mutase (EC 5.4.2.11) (Variovorax sp. SCN45)
MHKLVLIRHGESTWNLENRFTGWTDVDLTDTGVEQAKQAGRLLKAEGYDFDVAYTSVLKR
ATRTLWHTLDELDRTWLPVVHSWRLNERHYGGLQGLNKAETAKKYGDEQVLVWRRSYGTP
PPPLEANDPRSERSDVRYAKLSPEQIPLTECLKDTVARVLPFWNESMAPAIRAGRRLVVA
AHGNSIRALVKYLDGISDDAIVGLNIPNGIPLVYELDDDLKPLRSYYLGDAAAAEKAAAA
VASQGKG