Protein Info for GFF2561 in Sphingobium sp. HT1-2

Annotation: Glutamine synthetase adenylyl-L-tyrosine phosphorylase (EC 2.7.7.89) / Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 897 PF03710: GlnE" amino acids 10 to 234 (225 residues), 165.3 bits, see alignment E=2.7e-52 amino acids 499 to 739 (241 residues), 169.8 bits, see alignment E=1.1e-53 PF08335: GlnD_UR_UTase" amino acids 259 to 374 (116 residues), 87 bits, see alignment E=1.9e-28 amino acids 766 to 884 (119 residues), 28.8 bits, see alignment E=1.8e-10

Best Hits

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 83% identity to sjp:SJA_C1-26730)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (897 amino acids)

>GFF2561 Glutamine synthetase adenylyl-L-tyrosine phosphorylase (EC 2.7.7.89) / Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) (Sphingobium sp. HT1-2)
VTDWIDLEARIRAHSPFLARQMDRYPAVVALLATGDFDAAMAAAQAQGVPEDGVAKSLRR
RRGAIALVTAAADLAGAWDMDRVTRTLSDFADQALEEALAATFAERYPDAVPQGFVVLAL
GKHGSRELNYSSDIDPILLYDPTTLPHGEREDVADAAVRIGRRVSEILNARDGDGYVFRV
DLRLRPSPEATPIALPVEAAIGYYESTAMGWEQAAFIRARPAAGDMALGEYFLRQIRPFV
WRRSLDFGAIDAITDISRRIRDHYAQGQAFGPGYDLKRGRGGIREVEFFAQVHQLIHGGR
DPSLRSGHTRTALRALAAAGVIEAEVAARLDEAYILFRTIEHRLQMVEDRQTHELPKNPD
SLDNVARLHGLTDGAALLDLLRPHVGWVGANYDRLTAAPDDGSLSHDEDRLKAQLKEMGF
ADADWAAARVAHWRDGTIRSLRSGASREALERLLPVLMPAIATAPDPGRALNRLDDMIGR
LPSAINFFKLLGARPGLVELLAQILSHAPALADVLSRRVELLEGLIDASAFDPVPPVDVL
AQQFSALEAGEDYQALLDRVRQRVNDRRFALGVQIVRGGDPLEAGRGYGRVAEAAIEALA
GATVTEFESAHGKVPGGEMVILALGRMGGGVLTHASDLDLVYLFTGDFLAESDGARPLGA
TQYFNRLGQRITNALSVSTAAGPLYEVDTRLRPSGAQGLLAVSLDSFAKYQREEAWTWEH
LALTRARPVFGSAQARSALEAILTETLQRPRDFDELARQAVKMRGDIARHKPPASELDVK
LVPGGLVDIEFLIHISQFHYRLGFDPDLGEALAELVAAGHLPATLIPAQELITRFLIVSR
LVSPKSTEPPEATRPLVARACGAADWDALLESYAKARQSVGDAWRALAAPYQENQDA