Protein Info for PS417_13000 in Pseudomonas simiae WCS417

Annotation: isochorismatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00857: Isochorismatase
  • amino acids 25 to 201 (177 residues), 123.2 bits, E=6.6e-40

Best Hits

Swiss-Prot: 78% identical to NICF_PSEPK: Maleamate amidohydrolase (nicF) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K13995, maleamate amidohydrolase [EC: 3.5.1.107] (inferred from 98% identity to pfs:PFLU2796)

MetaCyc: 78% identical to maleamate amidohydrolase (Pseudomonas putida KT2440)
RXN-646 [EC: 3.5.1.107]

Predicted SEED Role

"N-carbamoylsarcosine amidase (EC 3.5.1.59)" (EC 3.5.1.59)

MetaCyc Pathways

• nicotinate degradation I (6/6 steps found)
• picolinate degradation (4/7 steps found)
• creatinine degradation II (2/5 steps found)
• nicotinate degradation II (2/5 steps found)
• nicotine degradation III (VPP pathway) (5/10 steps found)
• nicotine degradation II (pyrrolidine pathway) (5/11 steps found)
• superpathway of nicotinate degradation (6/18 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.107 or 3.5.1.59

Sequence Analysis Tools

See A0A1N7U9D9 at UniProt or InterPro

Protein Sequence (210 amino acids)

>PS417_13000 isochorismatase (Pseudomonas simiae WCS417)
MSEQSADANYQGVWGNRIGFGKKAALLMIDFMQGYTTEGAPLFAPGVVTAVAESVELLAT
ARLHGIAVVHTNIRYHPGHFVDGGIWVKKAPVMKDMVEGNPLAAFCAEVLPLDEEVVITK
QYASAFFGSSLAPMLHAQGIDTVVLAGCSTSGCIRATAVDAVQHGFRTIVVRECVGDRHP
APHEANLFDIDSKYGDVVSKQEAMRKFREQ