Protein Info for GFF2542 in Xanthobacter sp. DMC5

Annotation: Pyruvate, phosphate dikinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 885 TIGR01828: pyruvate, phosphate dikinase" amino acids 3 to 883 (881 residues), 1399.1 bits, see alignment E=0 PF01326: PPDK_N" amino acids 21 to 58 (38 residues), 37.3 bits, see alignment (E = 3.1e-13) amino acids 63 to 364 (302 residues), 136.3 bits, see alignment E=2.2e-43 PF00391: PEP-utilizers" amino acids 435 to 515 (81 residues), 86.6 bits, see alignment E=9.8e-29 PF02896: PEP-utilizers_C" amino acids 530 to 881 (352 residues), 265.1 bits, see alignment E=1.1e-82

Best Hits

Swiss-Prot: 69% identical to PPDK_RHIME: Pyruvate, phosphate dikinase (ppdK) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01006, pyruvate,orthophosphate dikinase [EC: 2.7.9.1] (inferred from 90% identity to azc:AZC_0410)

Predicted SEED Role

"Pyruvate,phosphate dikinase (EC 2.7.9.1)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.9.1

Use Curated BLAST to search for 2.7.9.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (885 amino acids)

>GFF2542 Pyruvate, phosphate dikinase (Xanthobacter sp. DMC5)
MTKWVYTFGDGKAEGKADMKNLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYYYAHGQT
YPAELKGEVEAALAQVGQLTGKAFGDAANPLLVSVRSGARASMPGMMDTVLNLGLNDATV
DAVARLADRRFAYDSYRRFITMYSDVVLGVDHHHFEEILEHYKLQHGHTLDTDLSADDWA
ALVVKYKAKVESQLGKPFPQDPHEQLWGAIGAVFSSWMNARAITYRRLHSIPESWGTAVN
VQSMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTEKARKA
ASSDKPSMEVALPEAFKEFERISHVLENHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKAA
LRIAVELAAEGLITEQEAVGRVDPAALDQLLHPAIDPKAERKVIATGLPASPGAAAGAIV
FSADEAEAWKEQGRKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKPCV
SGAGALRIDNAAQTLTVNGQTFKKGDLITIDGGTGQVLAGEVAMLQPELSGEFATLMGWA
DKVRKLKVRANAETPADARMARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGRR
SALAKLLPMQRDDFVELFEIMKGLPVTIRLLDPPLHEFLPHTAEETAEVAKSLGVDIERV
ERRYKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAVIAAKSTGEAVVPEVMVPLI
ATKAEFDIVDKVIRDTAKAVEQETGATLTFQVGTMIELPRAALRAEEIAKGAEFFSFGTN
DLTQTCFGVSRDDAGTFLGPYVQKGILEVDPFVSIDVDGVGELVKIGVERGRKSRPDIKL
GICGEHGGDPASVAFCHEVGLNYVSCSPFRVPIARLAAAQAALKK