Protein Info for GFF2533 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 941 PF03710: GlnE" amino acids 79 to 270 (192 residues), 149.6 bits, see alignment E=1.6e-47 amino acids 555 to 795 (241 residues), 192.5 bits, see alignment E=1.3e-60 PF08335: GlnD_UR_UTase" amino acids 301 to 441 (141 residues), 100.7 bits, see alignment E=1.1e-32 amino acids 833 to 906 (74 residues), 29 bits, see alignment 1.5e-10

Best Hits

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 70% identity to dia:Dtpsy_3369)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (941 amino acids)

>GFF2533 Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTASHFSGSTAEEPVLSSNVNPCASLTADDRRADHSRFVQRVRRRYAAELSCLPPGEPVL
ASMQTCLGLLRERGLPLPAALRVLRQLVLERLVVLDGEQGAPLAVITRAVTELAEFALDQ
ACALAFADLDELHGAPQFTTPQGALQRAQLWVIGMGKLGARELNVSSDIDLIYVYDQDGE
TAGNAQGRQRISNHEYFGKAVKHIYNTVGETTEHGNVFRVDLALRPNGNSGPSAVSLGAL
EEYFLVQGREWERFAWLKSRVIAPAACVQDRSASALRGTVLPFVFRRYLDYGVFDALRTL
HRQIRDHASKRAAGNPGRSNDVKLSRGGIREIEFTVQLLQVVRGGQFPELRTRPTLDALQ
RLCKAGLMPPATASALAQAYEFLRRVEHRIQYLDDQQTHVMPTRDDDLAWLAATLGYTDV
CAFLHALDAHRELVAQEFDTLLGGPTNGGCNGKGCSSKKNGASGRSAVDDLQGVLEQLPE
AFAARVAQWREHPRVLALREDTRARLVRLVQRTGAWIDEGRVSEEAALRMADWIEPLLRR
ESYLALLQERPSVHERLLRLLGAAKWPARYLIQHPGVIDELASQQLLTDRFDAAQFEDEL
ESRRTALRSTGEDDEEALLNLLRRAHHAEVFRTLARDVEKVLTVEQVADDLSALADSVLR
VTARWCWSRLKSRHRDEPSFAIIGYGKLGGKELGYGSDLDIVFVYEDEHENASEIYAAFV
RKMINWLTVKTGEGDLFEIXTALRPNGNSGLLVTSFTAYANYQQQRGSNTAWTWEHQAMT
RARFVLGGVSLAGRFDAVREAVITAERDAASLAAEIVAMRGKVRAAHPVRGERFDVKHSP
GGMVDVEFAVQYLVLLHSREHPELRANTGNINLLRRAENAGLLEPGMGEAAAHAYRELRQ
IQHRARLDEAPTQVDAAQVEEASRAVLALWQHVLGRATSTA