Protein Info for GFF2518 in Variovorax sp. SCN45
Annotation: Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to SYP_VARPS: Proline--tRNA ligase (proS) from Variovorax paradoxus (strain S110)
KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 97% identity to vpe:Varpa_4824)Predicted SEED Role
"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)
MetaCyc Pathways
- tRNA charging (20/21 steps found)
- pyoluteorin biosynthesis (1/7 steps found)
- ergotamine biosynthesis (4/12 steps found)
- (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
- clorobiocin biosynthesis (1/10 steps found)
- superpathway of ergotamine biosynthesis (5/18 steps found)
- pyrrolomycin biosynthesis (1/14 steps found)
- sulfazecin biosynthesis (1/16 steps found)
- phosalacine biosynthesis (4/25 steps found)
- phosphinothricin tripeptide biosynthesis (4/25 steps found)
- colibactin biosynthesis (6/38 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.1.1.15
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (605 amino acids)
>GFF2518 Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type (Variovorax sp. SCN45) MQALRVSPGEQFPTSIPVSITAIPMKASRFFVSTLKEAPADAEVASHRLMMRAGMIKKLG TGIYTYMPMGLRVIRKVEAIVREEMNRAGAVEMTMPVVQPAEFWQETGRFEKMGPELLRI KDRHDRDFVIQPTSEEVVTDIARQEIRSYKQLPKNFYQIQTKFRDERRPRFGLMRGREFI MKDAYSFDRDLDAAKASYQIMAQAYRNIFDRFGLRYRAVAADSGAIGGDLSEEFQVIAAT GEDAIVYCPDSDYAANMEKAEALAPAGPRPAAAKALEKTPTPGKSTCADVAELLGVPLST TIKSLVLATDILDAAGNPKGSQVWLLLLRGDHDMNEIKVSKLPGLDQGFRFATLAEIDEH FGCKPGYLGPLNLKKPVKLVADREAAVLADWITGANEVDFHMTGVNWGRDLPEPDLVADI RNVVAGDASPDGKGVLAIERGIEVGHVFVLGTKYSKDMNATYLDEGGKPQFLEMGCYGIG ITRLPAAAIEQNHDERGIIWPDALAPFTVVVCPIGMDRSPEVKVAAEALYEELLAKGVDV LLDDRGERPGAMFADWELIGVPHRVVISDRGLKEGQLEYQGRRDTAATKVPAAGIAEFIT GKLAK