Protein Info for GFF2515 in Variovorax sp. SCN45

Annotation: Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01027: glutamate 5-kinase
  • amino acids 5 to 367 (363 residues), 418.5 bits, E=1.2e-129
• PF00696: AA_kinase
  • amino acids 6 to 233 (228 residues), 153.4 bits, E=8.4e-49
• PF01472: PUA
  • amino acids 277 to 350 (74 residues), 67.5 bits, E=7.9e-23

Best Hits

Swiss-Prot: 96% identical to PROB_VARPS: Glutamate 5-kinase (proB) from Variovorax paradoxus (strain S110)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 97% identity to vpe:Varpa_4827)

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11)" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-ornithine biosynthesis II (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Protein Sequence (370 amino acids)

>GFF2515 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA (Variovorax sp. SCN45)
LRDARRIVVKVGSSLVTNEGRGLDEAAIGEWCRQLAELVRDGREVVMVSSGAIAEGMKRL
GWRTRPHEIHELQAAAAVGQMGLAQMYETKLRENQIGSAQVLLTHADLADRERYLNARST
LVTLLRLGVVPVINENDTVVNDEIKFGDNDTLGALVANLVEADALVILTDQKGLYTADPR
KDLEAKFVHEAAAGDPALEAMAGGAGSSIGKGGMITKILAAKRAAGSGASTVIAWGREAD
ALLRLAKGESIGTLLVAQTAKHQARKRWMADHLQLRGAVVVDAGAAAKVRAEGKSLLPIG
MTAVSGEFSRGDVIAVRDAEGVELARGLANYSSVEARLLCRKPSSEFERLLGYVAEPEMV
HRDNMVLMPS