Protein Info for GFF2514 in Variovorax sp. SCN45

Annotation: GTP-binding protein Obg

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02729: Obg family GTPase CgtA
  • amino acids 3 to 333 (331 residues), 465.7 bits, E=7.3e-144
• PF01018: GTP1_OBG
  • amino acids 4 to 158 (155 residues), 196.2 bits, E=3.9e-62
• PF01926: MMR_HSR1
  • amino acids 161 to 285 (125 residues), 88.1 bits, E=6.9e-29
• TIGR00231: small GTP-binding protein domain
  • amino acids 161 to 329 (169 residues), 49 bits, E=5.9e-17
• PF02421: FeoB_N
  • amino acids 162 to 329 (168 residues), 41.1 bits, E=2e-14

Best Hits

Swiss-Prot: 88% identical to OBG_ACIET: GTPase Obg (obg) from Acidovorax ebreus (strain TPSY)

KEGG orthology group: K03979, GTP-binding protein (inferred from 95% identity to vap:Vapar_4216)

MetaCyc: 56% identical to GTPase ObgE (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein Obg"

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (358 amino acids)

>GFF2514 GTP-binding protein Obg (Variovorax sp. SCN45)
MKFVDEAFIDIAAGDGGNGCVSFRHEKYKEFGGPNGGDGGRGGHVFAVADSNLNTLVDFR
YSRRHEAKRGEHGMGSDMFGAAGDDITLKMPVGTIISDAETGEVLFELLAEGEVITIAKG
GDGGFGNMRFKSAINRAPRQKTPGWPGEKRSLKLELKVLADVGLLGMPNAGKSTLISAIS
NARPRIADYPFTTLHPNLGVVRVGPEQSFVVADLPGLIEGASEGAGLGHLFLRHLQRTRL
LLHVVDMAPFDDSIDPVAQAKAIVGELKKYDAALYEKPRWLVLNKLDMVPADERAARVKD
FVKRLRFKGPVFEISALTREGCEHLVQAIYQQVKAQQVAEHEPVEIDPRFIPLPPEGD