Protein Info for GFF2513 in Xanthobacter sp. DMC5

Annotation: Multidrug resistance protein MdtN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 transmembrane" amino acids 7 to 24 (18 residues), see Phobius details PF13533: Biotin_lipoyl_2" amino acids 43 to 91 (49 residues), 30.4 bits, see alignment 4e-11 PF16576: HlyD_D23" amino acids 45 to 274 (230 residues), 37.4 bits, see alignment E=2.5e-13 PF13437: HlyD_3" amino acids 196 to 277 (82 residues), 43.8 bits, see alignment E=5.7e-15

Best Hits

Swiss-Prot: 31% identical to MDTN_SHIFL: Multidrug resistance protein MdtN (mdtN) from Shigella flexneri

KEGG orthology group: None (inferred from 44% identity to sno:Snov_0437)

MetaCyc: 31% identical to putative multidrug efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-351

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (326 amino acids)

>GFF2513 Multidrug resistance protein MdtN (Xanthobacter sp. DMC5)
LLSFVERHVVASILGAVVLAFVVYETSVRFFAYSGDAYVATDVIFIAPEVSGPLAKVVVG
DDAPVAAGAVLAEIERQPFALAVAAAEAQVTLAAERIRLAEAAIDESTAMVSAARASLDN
ARRQQTRSESLSQTGDISAEALDMVRRDAVVAAADLQKAESLAAVAQTLVTVRRAEQATA
AQALAKASYELSRTRIVSPVKGRVAPLTVRTGDYATAGKPLAAVVSDLDWHIVAAVDERH
LARLKPGQSVLFTIGSDPWRPHWGTVRSISPGIARSAGEDGALPYVPLSTDWIRLPRRFP
VLIDMGDLPARERLYRGADARVLIWF