Protein Info for PS417_12815 in Pseudomonas simiae WCS417

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 725 PF13185: GAF_2" amino acids 170 to 311 (142 residues), 30.1 bits, see alignment E=1.6e-10 PF01590: GAF" amino acids 172 to 306 (135 residues), 51.2 bits, see alignment E=6e-17 PF00512: HisKA" amino acids 362 to 424 (63 residues), 34.8 bits, see alignment E=4.3e-12 PF02518: HATPase_c" amino acids 468 to 587 (120 residues), 63.8 bits, see alignment E=5.8e-21 PF00072: Response_reg" amino acids 612 to 719 (108 residues), 56.1 bits, see alignment E=1.2e-18

Best Hits

KEGG orthology group: None (inferred from 88% identity to pfs:PFLU2765)

Predicted SEED Role

"Sensor histidine kinase/response regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U1F1 at UniProt or InterPro

Protein Sequence (725 amino acids)

>PS417_12815 diguanylate cyclase (Pseudomonas simiae WCS417)
MNWLQSSSDMAERVRLHDWASSPLGPLEQWPDVLKTTVALCLASSFPQSIVWGPQLTMLY
NDAFIPILGDKPYALGRPFSEVWHEAWADIGPIANAAFEGHATYIENFPLLIERGNGSEQ
AYFTFCYSPIRDPQGRVVGMLDTVTETTATVFLARRLAVLDAVGSAVANATDAETIMSTT
TRLLAEHLQVSNCAYADMDADEDGFTIRGNWAAPGSPSIVGRYSLASFGARAVRQLRAGE
PLVVEDNRVEFPPEEAASYQALDVLATVCFPLIKDGRLTALMAVHHKKARVWSPYDLALV
GEVTERSWAHIERVRADAAVREGLAAFTELNATLEQRVMERTAALTQAEAALHQSQKLEA
IGQLTGGVAHDFNNLLTIIRSSVDFLRQPGLSEERRQRYMSAVSDTVERASKLTSQLLAF
ARRQPLNPEVFDAGQRVKNIGEMLESVTGARIHVQVHLPEHPCHVRVDASQFETALINIA
LNARDAMEGQGTLTLRVVTVEAMPRIRGDAEARQPFVSISLADTGSGIPGDAVERIFEPF
FTTKAVGKGTGLGLSQVFGFAKQSGGNVEVVSTPGQGSVFTLYLPEATPQALPDEQPQQG
PAPTHDAAQRHILIVEDNLEVGRFATQILQDLGYQTTWATNAEQALALAGTDALAFDGVF
SDVVMPGMTGVAMAKVLRQRRADLPVVLTSGYSEELADSGYEGFEFLPKPYSADQVARAL
AKAMP