Protein Info for PS417_12800 in Pseudomonas simiae WCS417

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1115 TIGR02456: trehalose synthase" amino acids 16 to 561 (546 residues), 939.6 bits, see alignment E=5e-287 PF00128: Alpha-amylase" amino acids 24 to 475 (452 residues), 305.3 bits, see alignment E=1.5e-94 PF16657: Malt_amylase_C" amino acids 476 to 558 (83 residues), 89.7 bits, see alignment 1.6e-29 TIGR02457: putative maltokinase" amino acids 578 to 1099 (522 residues), 626.7 bits, see alignment E=3.3e-192 PF18085: Mak_N_cap" amino acids 610 to 670 (61 residues), 36.5 bits, see alignment 1.2e-12

Best Hits

KEGG orthology group: K05343, maltose alpha-D-glucosyltransferase [EC: 5.4.99.16] (inferred from 71% identity to avn:Avin_28000)

Predicted SEED Role

"Trehalose synthase (EC 5.4.99.16)" in subsystem Trehalose Biosynthesis (EC 5.4.99.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.16

Use Curated BLAST to search for 5.4.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U9A0 at UniProt or InterPro

Protein Sequence (1115 amino acids)

>PS417_12800 alpha-amylase (Pseudomonas simiae WCS417)
MAKKPNAATFIKDPLWYKDAVIYQVHVKSYFDSNNDGIGDFPGLIAKLDYIADLGVNTIW
LLPFYPSPRRDDGYDIAEYRGVHSDYGTMADAKRFIAEAHKRGLRVITELVINHTSDQHP
WFQRARKAKPGSAARDFYVWSDDDQKYDGTRIIFLDTEKSNWTWDPVAGQYFWHRFYSHQ
PDLNFDNPQVMKAVLSVMRYWLDMGIDGLRLDAIPYLIERDGTNNENLPETHDVLKQIRA
EIDAHYPDRMLLAEANQWPEDTQLYFGDKKGDDGDECHMAFHFPLMPRMYMALAQEDRFP
ITDILRQTPEIPANCQWAIFLRNHDELTLEMVTDKERDYLWNYYAADRRARINLGIRRRL
APLMERDRRRVELLNSLLLSMPGTPTLYYGDEIGMGDNIYLGDRDGVRTPMQWSIDRNGG
FSRADPASLVLPPIMDPQYGYPSVNVETQAQDPHSLLNWTRRMLAVRKQSKAFGRGSLKM
LSPSNRRILAYTREFTGEDGRNEIILCVANVSRSAQAAELDLSAFAGMVPVEMLGGNAFP
PIGQLNFLLTLAPYGFYWFVLAPENQMPSWHVEPAQSMPDFTTLVLKKRMEELLDEPCRA
SLEQTVLPAWLPKRRWFAGKDTAIDSVRIAYGVRFGDPQHPVLLSELEVTAGGQVSRYQL
PFGFLGEDQFTSALPQQLALARVRRVRQVGLVTDAFSLEHFVRTVIQGLQAGTLLNTHEG
ELRFAATPHLAKLQLADESEVRYLSAEQSNSSVVVGECLVLKLIRKVASGVHPELEMSAY
LTEAGYPNISPLLGSMIRHDAEGQDNLLMIAQGYLSNQGDAWGWTQNNLERAIRDELAEA
MSEQEQHYNALGELADFAGLLGQRLGEMHRVLGAKTTNKDFKPEVTTVKDTQAWAKDVGA
QLDRALQLLKLHQSQLNLADQALVSEMLAQKKAIASHVQALAKATAGGLRIRVHGDLHLG
QVLVVKGDAYLIDFEGEPARPLHERRGKHSPYKDVSGVLRSFDYAAAMALNVQGVDHSPE
ADQARKRVTDRYLKEARQAFIQAYQSATSTLAHDWQDAKGQDAALTLFSLEKAAYEVAYE
AENRPTWLPVPLQGLHGLLSGLAPISKTARGGETS