Protein Info for GFF2499 in Sphingobium sp. HT1-2

Annotation: Alkaline phosphatase (EC 3.1.3.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF01663: Phosphodiest" amino acids 44 to 480 (437 residues), 126.6 bits, see alignment E=2.7e-40 PF01676: Metalloenzyme" amino acids 274 to 350 (77 residues), 27.4 bits, see alignment E=3.6e-10

Best Hits

Swiss-Prot: 99% identical to ALPH_SPHSX: Alkaline phosphatase PhoK (phoK) from Sphingomonas sp.

KEGG orthology group: None (inferred from 82% identity to sch:Sphch_1382)

Predicted SEED Role

"Alkaline phosphatase (EC 3.1.3.1)" in subsystem Phosphate metabolism (EC 3.1.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.1

Use Curated BLAST to search for 3.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (559 amino acids)

>GFF2499 Alkaline phosphatase (EC 3.1.3.1) (Sphingobium sp. HT1-2)
MLKHVAAALLLATAMPVVAQSPAPAAAPAPAARSIAATPPKLIVAISVDQFSADLFSEYR
QYYTGGLKRLTSEGAVFPRGYQSHAATETCPGHSTILTGSRPSRTGIIANNWFDLDAKRE
DKNLYCAEDESQPGSSSDKYEASPLHLKVPTLGGRMKAANPATRVVSVAGKDRAAIMMGG
ATADQVWWLGGPQGYVSYKGVAPTPLVTQVNRAFAQRLAQPNPGFELPAQCVSKDFPVQA
GNRTVGTGRFARDAGDYKGFRISPEQDAMTLAFAAAAIESMQLGKQAQTDIISIGLSATD
YVGHTFGTEGTESCIQVDRLDTELGAFFDKLDKDGIDYVVVLTADHGGHDLPERHRMNAM
PMEQRVDMALTPKALNATIAEKAGLPGKKVIWSDGPSGDVYYDKGLTAAQRARVETEALK
YLRAHPQVQTVFTKAEIAATPSPSGPPESWSLIQEARASFYPSRSGDLLLLLKPRVMSIP
EQAVMGSVATHGSPWDTDRRVPILFWRKGMQHFEQPLGVETVDILPSLAALIKLPVPKDQ
IDGRCLDLVAGQGDSCAGQ