Protein Info for GFF249 in Xanthobacter sp. DMC5

Annotation: Acetone carboxylase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 776 PF02538: Hydantoinase_B" amino acids 54 to 631 (578 residues), 334.9 bits, see alignment E=4e-104

Best Hits

Swiss-Prot: 96% identical to ACXB_XANP2: Acetone carboxylase alpha subunit (acxB) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K10854, acetone carboxylase, alpha subunit [EC: 6.4.1.6] (inferred from 96% identity to xau:Xaut_3510)

MetaCyc: 96% identical to acetone carboxylase alpha subunit (Xanthobacter autotrophicus Py2)
Acetone carboxylase. [EC: 6.4.1.6]

Predicted SEED Role

"Acetone carboxylase, alpha subunit (EC 6.4.1.6)" (EC 6.4.1.6)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.6

Use Curated BLAST to search for 6.4.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (776 amino acids)

>GFF249 Acetone carboxylase alpha subunit (Xanthobacter sp. DMC5)
MNVNVKQTSLQGATRGIVRGGETLKEHRDRLMTATKATGHYAGLKKLELRESEPILYNKL
FSRLRAGVVDARETAKKIAASPIVEQEGELCFTLYNAAGDSLLTSTGIIIHVGTMGAAIK
YMIENNWEANPGVHDKDIFCNNDSLIGNVHPCDIHTIVPIFWEGELIGWVGGVTHVIDTG
AVGPGSMATGQVQRFGDGYSITCRKVGANDELFRDWLHESQRMVRTTRYWMLDERTRIAG
CHMIRKLVEEVVSEEGIEAYWKFAYEAVEHGRLGLQARIKAMTIPGTYRQVGFVDVPYAH
EDVRVPSDFAKLDTIMHAPCEMTIRKDGTWRLDFEGSSRWGWHTYNAHQVSFTSGIWVMM
TQTLIPSEMINDGAAYGTEFRLPKGTWMNPDDRRVAFSYSWHFLVSAWTALWRGLSRSYF
GRGYLEEVNAGNANTSNWLQGGGFNQYDEIHAVNSFECAANGTGATAVQDGLSHAAAIWN
PEGDMGDMEIWELAEPLIYLGRQIKASSGGSGKYRGGCGFESLRMVWNAKDWTMFFMGNG
HISSDWGLMGGYPAASGYRFAAHKTGLKELIASGADIPLGGDTDPENPTWDAMLPKAQIK
RDKQAITTEEMFSDYDLYLNYMRGGPGFGDPLDREPQAVADDINGGYVLERFAGDVYGVV
VRKGADGLVAVDEAATKAARAQIRKDRIAKSVPTREWMKGEREKILAKDAGTQVQQMFAA
SFKLGPRFEQQFRSFWDLPESWVLNEEDIGVPTYGSRYSMDISELPDVTTVQFVEE