Protein Info for PS417_12695 in Pseudomonas simiae WCS417
Annotation: mannitol dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 47% identical to M2DH_ASPFU: Mannitol 2-dehydrogenase (AFUA_4G14450) from Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
KEGG orthology group: K00045, mannitol 2-dehydrogenase [EC: 1.1.1.67] (inferred from 94% identity to pfs:PFLU2741)Predicted SEED Role
"Multiple polyol-specific dehydrogenase (EC 1.1.1.-)" in subsystem Mannitol Utilization or Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization (EC 1.1.1.-)
MetaCyc Pathways
- mannitol cycle (3/5 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of type II polyketide products
- Biosynthesis of unsaturated fatty acids
- Bisphenol A degradation
- Butanoate metabolism
- C21-Steroid hormone metabolism
- Fructose and mannose metabolism
- Glycine, serine and threonine metabolism
- Insect hormone biosynthesis
- Linoleic acid metabolism
- Nucleotide sugars metabolism
- Polyketide sugar unit biosynthesis
- Retinol metabolism
- Tetrachloroethene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.-
Use Curated BLAST to search for 1.1.1.- or 1.1.1.67
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7UHE8 at UniProt or InterPro
Protein Sequence (493 amino acids)
>PS417_12695 mannitol dehydrogenase (Pseudomonas simiae WCS417) MKLNKQNLAQLALQVKLPTYEIASTTQGIAHIGVGGFHRAHQAYYTDALMNTGVGLDWSI CGVGLRAEDRKARDDLAGQDYLFTLYELGDTDDTEVRVIGSISDMLLAEDGAQALIDKLA DPAIRIVSLTITEGGYCIDDSNGEFMAHLPQIQHDLAHPSAPKTVFGFICAALTKRRAAG TPAFTVMSCDNLPHNGAVTRKALLAFAALHNAELHDWIKANVSFPNAMVDRITPMTSTAH RLQLHDDHGIDDAWPVVCEPFVQWVLEDKFVNGRPAWETVGVQFTDDVTPYEEMKIGLLN GSHLALTYLGFLKGYRFVHETMNDPVFVAYMRAYMDLDVTPNLAPVPGIDLTDYKQTLVD RFSNQAIADQLERVCSDGSSKFPKFTVPTINRLIADGRETERAALVVAAWALYLKGVDEN GVSYKIPDPRAEFCQGLVSDDALISQRLLAVEEIFGTAIPNSPGFVAAFERCFVSLRDNG VTATLQHLLKKPN