Protein Info for GFF2481 in Variovorax sp. SCN45

Annotation: Urease accessory protein UreF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 222 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF01730: UreF" amino acids 39 to 179 (141 residues), 134.5 bits, see alignment E=1.9e-43

Best Hits

Swiss-Prot: 66% identical to UREF_POLSJ: Urease accessory protein UreF (ureF) from Polaromonas sp. (strain JS666 / ATCC BAA-500)

KEGG orthology group: K03188, urease accessory protein (inferred from 88% identity to vpe:Varpa_4862)

Predicted SEED Role

"Urease accessory protein UreF" in subsystem Urea decomposition

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (222 amino acids)

>GFF2481 Urease accessory protein UreF (Variovorax sp. SCN45)
VSAQSLLQLIWLASPALPVGGFSYSEGVEAAVEWAGVDSEPRAAEWLADQLHLSFARGDL
AVFAQAVQAWRANDTKRVRQLNDWVLATRESAEFFLQTEQMGRSFVEWLKLHHADTAQVF
ADLPASYPIAFAFAASRTDATVHDCCLAFAFGWAENMVAAAVKAVPLGQSAGQRILGRLS
NEIPEAVTRAIALGDDERQAFAPLLAVLSARHETQYSRLFRS