Protein Info for GFF2478 in Xanthobacter sp. DMC5

Annotation: D-glycero-alpha-D-manno-heptose 7-phosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 PF00288: GHMP_kinases_N" amino acids 91 to 174 (84 residues), 58.8 bits, see alignment E=5e-20 PF08544: GHMP_kinases_C" amino acids 251 to 329 (79 residues), 45.3 bits, see alignment E=9.3e-16

Best Hits

Swiss-Prot: 48% identical to HDDA_ANETH: D-glycero-alpha-D-manno-heptose 7-phosphate kinase (hddA) from Aneurinibacillus thermoaerophilus

KEGG orthology group: K07031, (no description) (inferred from 68% identity to cse:Cseg_1877)

MetaCyc: 48% identical to D-glycero-alpha-D-manno-heptose-7-phosphate kinase (Aneurinibacillus thermoaerophilus)
RXN-11364 [EC: 2.7.1.168]

Predicted SEED Role

"D,D-heptose 7-phosphate kinase" in subsystem Capsular heptose biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.168

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (349 amino acids)

>GFF2478 D-glycero-alpha-D-manno-heptose 7-phosphate kinase (Xanthobacter sp. DMC5)
MAIGDALNVRARAPLRLGLAGGGTDLSPYCDEHGGNVLNATIDRFAYAHIRVNRIGALVL
RARDVGTEEVLEPSLDHPITEGLMLHRAVYTHMMKTYCDGVALPLTMSTTIDVPAGSGLG
ASSALVVALIEAFTYALGLPLGPYDVAKLAFDIERKQLGLAGGRQDQYSAAFGGLNFIEF
LPSDRVIVNPLRIRREHLNEFEASLVICFSGQSRRSDTIIKEQVAGLKQMDAKTIENMHK
LKQDAIDMKLALLRGDIRATADILGRSWTSKKATAAGIATEQVDKLYDIAIASGAWAGKV
SGAGGGGFLMFLTDPENRYQLIGALNEAGGLASAVKFSMDGAEGWPIGI