Protein Info for GFF2477 in Sphingobium sp. HT1-2

Annotation: Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 242 signal peptide" amino acids 5 to 6 (2 residues), see Phobius details transmembrane" amino acids 7 to 29 (23 residues), see Phobius details amino acids 50 to 71 (22 residues), see Phobius details amino acids 87 to 108 (22 residues), see Phobius details amino acids 147 to 178 (32 residues), see Phobius details PF04116: FA_hydroxylase" amino acids 95 to 230 (136 residues), 109.8 bits, see alignment E=7e-36

Best Hits

KEGG orthology group: None (inferred from 85% identity to sjp:SJA_C1-28960)

MetaCyc: 79% identical to zeaxanthin 2,2'-beta-hydroxylase (Sphingomonas elodea)
1.14.15.-; 1.14.13.-,1.14.13.-; 1.14.13.-; 1.14.13.-

Predicted SEED Role

"Sterol desaturase family protein"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (242 amino acids)

>GFF2477 Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase) (Sphingobium sp. HT1-2)
MVAAILLSALAMTLIVGVRYLIASGGFALATRLRQPGLYRGQGRQIGREIGWSLASAAIY
GIPAGVVAWGWQVRGWTRIYEDVGRYPLWYLPVSVLLYLAAHDTWFYWTHRWMHAPRLFR
IAHAVHHASRPPTAWAAMSFHPWEALTGAVVIPALVFLIPIHVGALGVVLSIMTIMGVSN
HMGWEMFPRWMVRGPIGRWLITASHHQRHHEQYRCNYGLYFRVWDRLCGTDRGLGEFREK
QV