Protein Info for GFF2473 in Xanthobacter sp. DMC5

Annotation: NAD(P) transhydrogenase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 34 to 52 (19 residues), see Phobius details amino acids 58 to 77 (20 residues), see Phobius details amino acids 89 to 111 (23 residues), see Phobius details amino acids 131 to 151 (21 residues), see Phobius details amino acids 168 to 186 (19 residues), see Phobius details amino acids 192 to 211 (20 residues), see Phobius details amino acids 218 to 237 (20 residues), see Phobius details amino acids 242 to 262 (21 residues), see Phobius details PF02233: PNTB" amino acids 8 to 464 (457 residues), 656.5 bits, see alignment E=1.3e-201

Best Hits

Swiss-Prot: 72% identical to PNTB_RHORU: NAD(P) transhydrogenase subunit beta (pntB) from Rhodospirillum rubrum

KEGG orthology group: K00325, NAD(P) transhydrogenase subunit beta [EC: 1.6.1.2] (inferred from 94% identity to xau:Xaut_1950)

Predicted SEED Role

"NAD(P) transhydrogenase subunit beta (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (468 amino acids)

>GFF2473 NAD(P) transhydrogenase subunit beta (Xanthobacter sp. DMC5)
MTANLAALLYLVAGILFVLALRGLSHPTTSRQGNLFGMVGMGIAILTTLALSPPSDGIGW
VLVLAGIGIGGGVGAVVAKRIAMTAMPQLVAAFHSLVGLAAVLVAAAALYAPRAFGIGDP
GHLHGASLVEMSLGVAIGAITFTGSIIAFAKLNGNMSGKPIMLPARHLINGGLALLLLIL
IVAFVLTGSITLFWIITLLSLILGGLLIIPIGGADMPVVVSMLNSYSGWAAAGIGFTLGN
TALIITGALVGSSGAILSYIMCKGMNRSFISVILGGFGGDAAAAGAAGHAEARPVKQGSA
DDAAYIMKNAEKVIIVPGYGMAVAQAQHALREMADLLKEEGVEVKYAIHPVAGRMPGHMN
VLLAEAQVPYDEVFELEDINSEFAQADVAFVIGANDVTNPAAKTDPQSPIYGMPILDVEK
AKTVLFIKRGMSAGYAGVENELFFKDNTMMLFGDAKKVTEDVVKAFGH